EVALUATION OF ANTIINFLAMMATORY EFFECT OF Capparis Aphylla Roth. USING EXPERIMENTAL ANIMAL MODELS

Pharma courses

Pharma Admission

pharma courses

pharma admission



E.     Serum Rheumatoid Factor (RF)
A significant reduction in RF was observed in indomethacin, MECA 190 mg/kg, MECA 240 mg/kg) and MECA 300 mg/kg treated group as compared to model control group  (Fig 4.4.5).


Figure :Effect of methanolic extract of Capparis aphylla Roth. on serum rheumatoid factor.

Values are expressed as mean ± SEM. N=6.Values are statistically evaluated using ANOVA analysis followed by Dunnett’s test. Significant values were compared with #P<0.05 normal control Vs model control & *P<0.05 model control Vs all MECA groups

F. Serum C-Reactive Protein (CRP)
A significant reduction in CRP was observed in indomethacin, MECA 190 mg/kg, MECA 240 mg/kg  and MECA 300 mg/kg treated group as compared to model control group.  (Fig4.4.6)


Figure : Effect of methanolic extract of Capparis aphylla Roth.on serum CRP.

Values are expressed as mean ± SEM. N=6.Values are statistically evaluated using ANOVA analysis followed by Dunnett’s test. Significant values were compared with #P<0.05 normal control Vs model control & *P<0.05 model control Vs all MECA groups

G.    Serum albumin globulin ratio (A/G ratio) :
There was a significant decrease  in total protein albumin level, A/G ratio but significant increase in the level of the globulin by indomethacin in CFA inflammatory rats. the above change were brought back to near normal levels by MECA 190 mg/kg, MECA 240 mg/kg, MECA 300 mg/kg. Fig (4.4.7).


Figure :Effect of methanolic extract of Capparis aphylla Roth. on serum A/G ratio.

Values are expressed as mean ± SEM. N=6.Values are statistically evaluated using ANOVA analysis followed by Dunnett’s test. Significant values were compared with #P<0.05 normal control Vs model control & *P<0.05 model control Vs all MECA groups

H.    Effect of MECA on radiological changes of control and experimental animals  [IJPD, CURATIVE EFFECT ON RA]
Bone destruction, which is a common feature of adjuvant  arthritis, was examined by radiological analysis. Freund’s Complete Adjuvant treated rats had developed definite joint space narrowing of the intertarsal joints, diffuse soft tissue swelling that included the digits, diffuse demineralization of bone, marked periosteal thickening, and cystic enlargement of bone and extensive erosions produced narrowing or pseudowidening of all joint spaces and bending of phalangeal joints. In contrast, in rats treated with MECA  attenuate abnormalities consisted of asymmetric soft tissue swelling and small erosions, periosteal thickening, and minimal joint space narrowing, predominantly localized to the proximal areas of the paws. The drug control rats showed the normal architecture of phalangeal joints as that of control rats.

I.       Effect of MECA on histopathological changes of control and experimental animals[IJPD, CURATIVE EFFECT ON RA]
As shown in Fig  5 a  [NC]:Histology of synovial joint of normal control rat with intact morphology of synovium and synovial lining Fig  5 b [MC]: FA induced disease control rat showed plenty of lymphocytic infiltration [↓]in synovial lining with severe inflammation and marked angiogenesis [?] studied with proliferation of synovial cells [↓↓]Fig 5 c [SD]]: indomethacin 100mg/kg treated rats showed significant protection with mild lymphocytic infiltration [↓] with no evidence of thickening of synovial lining and angiogenesis Fig 5 d MECA 190mg/kg: MECA 190mg/kg treated rats showed milder angiogenesis [?], lymphocytic infiltration [↓] and synovial lining thickening[↓↓]. Fig 5 e MECA 240 mg/kg: MECA 240 mg/kg treated rats showed milder angiogenesis [?], synovial lining thickening [↓↓] with no evidence of lymphocytic infiltration. Fig 5 f MECA 300mg/kg: MECA 300mg/kg treated rats sho


 

Pages

FIND MORE ARTICLES