DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF LAMOTRIGINE AND CLOZAPINE IN SYNTHETIC MIXTURE BY ABSORPTION CORRECTION METHOD
ROBUSTNESS AND RUGGEDNESS
• Robustness and Ruggedness of the method was determined by subjecting the method to slight change in the method condition, individually, the:
• Change in Wavelength
LAMO at 306.80nm and 307.20 and CLO at 359.80nm and 360.20nm
• Change in instrument (UV-Vis Spectrophotometer model 1800 and 2450),
• Three replicates were made for the concentration (1, 3, 5 μg/ml of LAMO and 6, 18, 30 μg/ml of CLO) with different stock solution preparation.
Table 8: Robustness and Ruggedness data of LAMO and CLO*(n=3)
|
Conc. (μg/ml) |
Lamotrigine (Mean* ±% RSD) (n=3) |
|||||
|
Instrument |
Wavelength |
|||||
|
UV-2450 |
UV-1800 |
306.80 |
307.20 |
|||
|
1 |
0.216±0.75 |
0.217±0.57 |
0.214±0.38 |
0.216±0.75 |
||
|
3 |
0.684±0.38 |
0.685±0.29 |
0.683±0.23 |
0.686±0.23 |
||
|
5 |
1.105±0.14 |
1.106±0.15 |
1.106±0.11 |
1.105±0.15 |
||
|
Clozapine (Mean* ±% RSD) (n=3) |
||||||
|
|
UV-2450 |
UV-1800 |
359.80 |
360.20 |
||
|
6 |
0.053±0.88 |
0.055±0.85 |
0.052±0.89 |
0.054±0.86 |
||
|
| ||||||
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