DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF LAMOTRIGINE AND CLOZAPINE IN SYNTHETIC MIXTURE BY ABSORPTION CORRECTION METHOD

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ROBUSTNESS AND RUGGEDNESS
• Robustness and Ruggedness of the method was determined by subjecting the method to slight change in the method condition, individually, the:
• Change in Wavelength
LAMO at 306.80nm and 307.20 and CLO at 359.80nm and 360.20nm
• Change in instrument (UV-Vis Spectrophotometer model 1800 and 2450),
• Three replicates were made for the concentration (1, 3, 5 μg/ml of LAMO and 6, 18, 30 μg/ml of CLO) with different stock solution preparation.

Table 8: Robustness and Ruggedness data of  LAMO and CLO*(n=3)

Conc. (μg/ml)

Lamotrigine (Mean*  ±% RSD) (n=3)

Instrument

Wavelength

UV-2450

UV-1800

306.80

307.20

1

0.216±0.75

0.217±0.57

0.214±0.38

0.216±0.75

3

0.684±0.38

0.685±0.29

0.683±0.23

0.686±0.23

5

1.105±0.14

1.106±0.15

1.106±0.11

1.105±0.15

Clozapine (Mean*  ±% RSD) (n=3)


UV-2450

UV-1800

359.80

360.20

6

0.053±0.88

0.055±0.85

0.052±0.89

0.054±0.86


 

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