Antioxidant Activity of Flowers and Leaves of Tabernaemontana Divaricata Linn.

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About Author: C. Stalin*, S. Kamalasivam, E. Hariharan,
Pallavan College of Pharmacy,
Kancheepuram, Tamilnadu.

Abstract:
Tabernaemontana divaricata a common garden plant in tropical countries has been used as a traditional medicine. However, no recent review articles of T. divaricata, particularly discussing its pharmacological properties, are available. In present study, the antioxidant activity of ethanolic extract of flowers and leaves of Tabernaemontana divaricata linn were evaluated . Free radicals have been regarded as a fundamental cause of different kinds of diseases. They cause biochemical damage in cells and tissues, which result in several diseases such as arteriosclerosis, ischemia-reperfusion injury, liver disease, diabetes mellitus, inflammation, renal failure, aging, cancer, etc. Compounds that can scavenge free radicals are thus effective in ameliorating the progress of these related diseases. T.divaricata L. known locally as ‘Nandiyavattom’ belonging to the family, Apocynaceae a common plant in India, profoundly used in Ayurvedic medicine as  Anthelmintic, Anti-inflammatory, Anodyne, Diuretic property.1 The objective of the present study was designed to investigate the antioxidant efficacy of ethanolic extract of leaves and flower of Tabernaemontana divaricata (TALE & TLFE). Invitro antioxidant activity such as Reducing power ability ,  NBT reduction assay, Deoxyribose degradation assay was performed and was concluded that the ethanolic extract of leaves and flowers of Tabernaemontana divaricata showed significant antioxidant activity.

Reference ID: PHARMATUTOR-ART-1127

Introduction
Free radicals have been regarded as a fundamental cause of different kinds of diseases. They cause biochemical damage in cells and tissues, which result in several diseases such as arteriosclerosis, ischemia-reperfusion injury, liver disease, diabetes mellitus, inflammation, renal failure, aging, cancer, etc. The main characteristic of an antioxidant is its ability to trap free radicals. Highly reactive free radicals and oxygen species are present in biological systems from a wide variety of sources. These free radicals may oxidize nucleic acids, proteins, lipids or DNA and can initiate various types of degenerative diseases.6 T.Divaricata L (Family:Apocynaceae).Commonly calledas Nandiyavattom found in the dry regions of Indiaand Sri Lanka.The beneficial properties of T. divaricata are, anti-infection, anti-tumor action, analgesia and the enhancement of cholinergic activity in both peripheral and central nervous systems. The augmentation of cholinergic function may be of therapeutic benefit for many neurodegenerative diseases, particularly myasthenia gravis and Alzheimer's disease.2 chemical constituents include alkaloids and non-alkaloid constituents such as terpenoids, steroids, flavonoids, phenyl propanoids, phenolic acids and enzymes which played an important role in bringing out the activity.

OBJECTIVE:
T.Divaricata (Linn.)a much branched shrub with smooth brown bark and closely pubescent brachlets have been reported for various pharmacological activities to treat diseases like Anthelmintic, Anti-inflammatory, Anodyne, Diuretic property. An attempt was made to correlate these activities with generation of free radicals and to evaluate the selected plant for its antioxidant potential.2

EXPERIMENTAL METHODS
Fresh leaves and flowers of the plant were collected from Coimbatore district, Tamilnadu, India during the month of July, 2010. Theplant was identified and authenticated by the Dr. G.V.S. Murthy, Joint Director, Botanical Survey of India (BSI), Tamilnadu Agricultural University campus, Coimbatore.

Extract preparation:
The leaves and flowers of Tabernaemontana divaricatawere collected, thoroughly dried under shade and powdered mechanicallyand sieved through No.20 mesh sieve. The finely powdered leaves and flowers were kept in an airtight container until the time of use.3

Extraction procedure for leaf
The extraction was carried out by cold maceration and mechanical shaking method. The solvent used was 95% ethanol. About 60 g of powder was soaked with 600ml of 95% ethanol for 12 h and then macerated at room temperature using a mechanical shaker for 4 h. The extract was filtered off and the marc was again soaked with the same volume of 95% ethanol for 12 h and then further extracted for 4h and filtered. The filtrates were then combined concentrated under reduced pressure and evaporated at 400C. The percentage yield of the T.Divaricata Leaf extract (TALE) was 27.4%.3

Extraction procedure for flower
The extraction was carried out by continuous hot percolation method using Soxlet apparatus. The solvent used was 95% ethanol. About 50 g of powder was extracted with 400 ml of solvent. The extract was concentrated to dryness under controlled temperature between 40-50ºC. The percentage yield of the T.Divaricata flower extract (TLFE) was 18.2%.3

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