You are hereArticles

Articles


ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF OLMESARTAN MEDOXOMIL AND CILNIDIPINE IN THEIR COMBINED PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD

{ DOWNLOAD AS PDF }

About Authors:
Nehal C. Ghelani*, Ketan Dadhania, Shital Faldu
Department of Quality Assurance,
Smt. R. D. Gardi B. Pharmacy College, Rajkot, Gujarat, India
*nehalghelani10@gmail.com

Abstract
RP-HPLC method was developed for the simultaneous estimation of Olmesartan Medoxmil and Cilnidipine in pharmaceutical dosage form. The separation was achieved Hypersil C18 (250 x 4.6 mm, 5 mm) columnwith Acetonitritle:Phosphate buffer pH 3.6 (70:30 %v/v). Flow rate was maintained at 1.0 ml/ min and UV detection was carried at 270 nm. Retention time for Olmesartan Medoxomil and Cilnidipine was found to be 4.14 min and 7.79 min respectively. The method has been validated for linearity, accuracy and precision. Linearity for Olmesartan Medoxomil and Cilnidipine were in the range of 40 - 200 µg/ml and 10 - 50 µg/ml respectively. The percentage recoveries obtained for Olmesartan Medoxomil and Cilnidipine were found to be in range of 99.96- 100.23 and 98.99-100.03 respectively. The developed method was validated as per ICH guidelines. Developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of Olmesartan Medoxomil and Cilnidipine  in pharmaceutical dosage form.


ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL AND PROPYPHENAZONE IN THEIR COMBINED PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD

{ DOWNLOAD AS PDF }

About Authors:
Mehul Kakdiya*1, Viral M. Kakadiya2, Darshan Madiya1, Shital Faldu1
1Department of Quality Assurance, Smt. R. D. Gardi B. Pharmacy College, Rajkot, Gujarat, India
2Pacific college of pharmacy, Udaipur, Rajasthan
*kakdiyamehul@gmail.com

Abstract
A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Paracetamol and Propyphenazone in their combined pharmaceutical dosage form. The method was carried out on a Inertsil ODS 3V (250 x 4.6 mm i.d., 5 μ) column with a mobile phase consisting of acetonitrile: water in the ratio of 70:30 v/v at a flow rate of 1.0 mL/min. Detection was carried out at 238 nm.

The retention times of Paracetamol and Propyphenazone were 5.87 and 13.63min, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of Quantitation. The proposed method can be used for the estimation of these drugs in routine quality control.


ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMILORIDE AND TORSEMIDE IN THEIR COMBINED PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD

{ DOWNLOAD AS PDF }

About Authorts:
Krutika J. Bhalodiya*, Darshan Modiya, Shital Faldu
Department of Quality Assurance
Smt. R. D. Gardi B. Pharmacy College, Rajkot, India
bhalodiyakruti1@gmail.com

Abstract
RP-HPLC method was developed for the simultaneous estimation of Amiloride and Torsemide in pharmaceutical dosage form. The separation was achieved by BDS C18 (150 × 4.6 mm, 5 µm) column with Methanol:Phosphate buffer pH 3.6 (10:90 %v/v). Flow rate was maintained at 1.0 ml/ min and UV detection was carried at 288 nm. Retention time for Amiloride and Torsemide was found to be 1.944 min and 8.903 min respectively. The method has been validated for linearity, accuracy and precision. Linearity for Amiloride and Torsemide were in the range of 3-7 µg/ml and 6-14 µg/ml respectively. The percentage recoveries obtained for Amiloride and Torsemide were found to be in range of 99.87- 100.80 and 100.1-101.3 respectively. The developed method was validated as per ICH guidelines. Developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of Amiloride and Torsemide  in pharmaceutical dosage form.


A REVIEW ON ROLE OF HUMAN PAPILOMMA VIRUS(HPV) IN CERVICAL CANCER

{ DOWNLOAD AS PDF }

About Authors:
M.Sushma*, B.Vamsikrishna, M. Babu, R. Mohanraj
Department of Pharmacy Practice,
Raghavendra Institute of Pharmaceutical Education & Research (RIPER),
Anantapuram, Andhra Pradesh, INDIA
sushma.banthi@gmail.com

Abstract
Cervical cancer is a disease in which cancer cells form in the tissues of a woman's cervix. Globally, cervical cancer is the second most common cancer in women, and the fifth deadliest. Most common symptoms are increased vaginal discharge, Pelvic pain, Pain during sex. There are 4 stages of cervical cancer stage I, II, III, IV based on stage treatment is given. Diagnosis is done by pap smear, cone biopsy and cervical examining is done by colposcopy. Treatment options for women with cervical cancer are surgery, radiation therapy, Chemotherapy, and combination of these methods. Cervical cancer can be prevented by getting the HPV vaccine. Two types of vaccines are approved, Gardasil and Cervarix. They prevent against most types of HPV infection that cause cervical cancer, also Practice safer sex. Using condoms during sex reduces the risk of HPV and other sexually transmitted infections (STIs).


FORMULATION AND EVALUATION OF CONTROLLED RELEASE TABLETS OF METOPROLOL SUCCINATE BY - OSMOTIC DRUG DELIVERY

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
Deepthi.P*, Samatha. M, N. Srinivas
Department of Pharmaceutics,
Mallareddy Institute of Pharmaceutical Sciences, Secunderabad
deepthisairi@gmail.com

ABSTRACT
Osmotically controlled drug delivery systems utilize the principle of osmotic pressure for the controlled delivery of active agents. The release rate of the drug from these systems is independent of the physiological factors of the gastrointestinal tract to a large extent. Metaprolol succinate β-receptor blocking agent was selected as a model drug to be formulated into osmotic drug delivery system. Elementary osmotic pump core tablets for Metaprolol succinate non- aqueous wet granulation were prepared by using osmogens like KCl. The coated tablets were drilled to different orifice sizes by using mechanical microdrill by mechanical means using softclix - modified sharp needle. The drilled orifice sizes on coated tablets were evaluated by using scanning ocular micrometer. It was observed that with an increase in osmogen content and pore size, rate of drug release was found to be increasing. The rate of drug release was found to be decreased with an increase in the membrane thickness. Based on different experimental trials, the optimized formulation was selected with respective to osmogen concentration, membrane thickness and orifice size that are following zero-order controlled release from the elementary osmotic pump tablets. The final selected elementary osmotic pump tablets have shown comparable dissolution profile with respective to marketed formulation.


A COMPARATIVE STUDY FOR SAFETY AND EFFICACY OF OXACEPROL AND DIACEREIN IN OSTEOARTHRITIS OF KNEE JOINTS

{ DOWNLOAD AS PDF }

ABOUT AUTHOR:
Jay Shah1, Nazima Mirza2, Vivek Patel3
1Department of Clinical Pharmacy, A.R.College of Pharmacy and G.H.Patel Institute of Pharmacy,
Vallabh Vidyanagar, Gujarat, India.
2Department of Pharmacology, Pramukh Swami Medical College, Karamsad, Gujarat
3Department of Orthopedic, Shree Krishna Hospital, Karamsad, Gujarat
shahjayg@yahoo.com

ABSTRACT
Objectives: Osteoarthritis (OA) is the most common form of arthritis which has a growing number of patients and having major impact on the quality of life. According to modes of action the drugs for OA are divided into two groups. First group, NSAIDs which provide symptomatic relief but have no influence on progress of the disease and shows higher adverse events. The second group of drugs acts as disease modifying agents. Recent data from clinical trials have demonstrated that agents like Diacerein & Oxaceprol specifically block key disease mechanisms & effectively retard the progression of structural changes in knee OA patients. The study was undertaken to compare the efficacy & safety of Oxaceprol and Diacerein in the patients of Osteoarthritis of Knee joints.
Experimental/ Computational work done: Total 60 patients(4 withdrawn) suffering from mild to moderate OA of knee joint were included from outpatient orthopedic department of Shree Krishna hospital, Karamsad. Twenty eight patients received diecerein(50 mg b.i.d.) and 28 patients were given Oxaceprol(200 mg t.i.d.)  for 10 days and patient responses were measured using VAS and Lequesne scale, before and after drug treatment. Suspected adverse event was evaluated by filling ADR form. Ethical committee approval and patient consents were obtained prior to the beginning of the study.
Results and discussion: The VAS Scale was reduced from 7.321 ± 0.9449 to 4.75 ± 1.146 (Mean±SD) for Diacerein and 6.821 ±1.1564 to 4.321±1.1564 for Oxaceprol. The Lequesne scale was reduced from 6.0±1.054 to 4.21±1.0923 for Diacereine and 6.0±0.8819 to 3.839±1.0475 for Oxaceprol. Both the results were found to be statistically significant. Difference in VAS and Lequesne scale between the Diacerein and Oxaceprol were 0.757 and 0.099 respectively (p >0.05). There was not any Adverse Event reported.
Conclusions: Both the drugs, Diacerein and Oxaceprol, individually and equally effective in Osteoarthritis condition. Both Diacerein and Oxaceprol are found to be safer.


REVIEW ON HYDROGEL- A NOVEL CARRIER

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
C. Mallikarjuna*1, V. Hari Bhaskar1, Junju. Mohan Kumar2, Rayaprolu. Mounica2, Sai Padmini Bolla2
1Department of Pharmaceutics, Vagdevi College Of Pharmacy And Research Centre, SPSR Nellore, A. P, India.
2Department of Pharmaceutics, Rao’s College Of Pharmacy, SPSR Nellore, A. P, India.
Mallikarjuna2055@gmail.com

ABSTARCT
Hydrogels are three–dimensional cross-linked hydrophilic polymers that swell in water and aqueous solutions without dissolving in them. Softness, smartness, and the capacity to store water make hydrogels unique materials.Several techniques have been reported for the synthesis of hydrogels like co-polymerization/crosslinking of co-monomers using multifunctional co-monomer, which acts as crosslinking agent. They can be classified in different ways on the basis of their preparation, biodegradable properties, polymer, sensitivity to surrounding environment and also their application. Hydrogels being biocompatible materials have been recognized to function as drug protectors, especially for peptides and proteins, from in-vivo environment. Hydrogels that are responsive to specific molecules, such as glucose or antigens, can be used as biosensors as well as drug delivery systems. This review mainly deals with the advantages, properties, method of preparation and characterization of hydrogels.


REGULATORY AFFAIRS: “STUDY REPORT OF NEW DRUG REGISTRATION PROCESS IN EUROPEAN UNION”

{ DOWNLOAD AS PDF }

About Authors:
Yogeshkumar B. Viradiya*, Manoj B. Dagwar, Swapnil T. Lanjewar
Institute of Management Sciences and Research (IMSR),
Nagpur, Maharashtra
viradiya2210@gmail.com

Abstract:
European Union is the big market for pharmaceuticals. Every pharmaceutical company wants to starts their business in European Union. In European Union come different countries like UK, Germany, France, Ireland, Sweden etc. In these countries various different process for new drug registration process like Nationalize process, Centralize process, Decentralize process, Mutual recognition process. Before that clinical trial approval also important for new drug registration. New drug registration process in European Union takes approximately 33 to 35 weeks. Each company must follow the rules and regulation for new drug registration. European medicine agency which gives the Clinical trial authorization and Marketing authorization to the new drug.


DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF TRIAMTERENE AND BENZTHIAZIDE IN TABLETS

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
VC Chauhan*, VN Shah, DA Shah, RR Parmar
Department of Quality Assurance
APMC College of Pharmaceutical Education and Research,
Motipura, Himmatnagar, Gujarat 383001
vikas14vks@gmail.com

ABSTRACT
A  specific,  accurate,  precise  and  reproducible  RP-HPLC  method  has  been  developed  and subsequently validated for the simultaneous determination of Triamterene and Benzthiazide in tablets. The proposed HPLC method utilizes BDS hypersil (Thermo scientific) C18 column (250 mm × 4.6 mm id, 5 μm particle size), and mobile phase consisting of phosphate buffer: methanol (70:30) and pH adjusted to 3.5 with sodium hydroxide and flow rate of 1.0 ml/min. Quantitation was achieved with UV detection at 245 nm based on peak area with linear calibration curves at concentration ranges 10-30 μg/ml for Triamterene and 5-15 μg/ml for Benzthiazide. The retention time of Triamterene and Benzthiazide were found to be 5.960 min and 3.493 min respectively.  The  method  was  validated  in  terms  of  accuracy,  precision,  linearity,  limits  of detection,  limits  of  quantitation  and  robustness.  This  method  has  been  successively  applied  to tablet formulation  and  no interference  from the  formulation excipients  was found.


PREPARATION AND EVALUATION OF MATRIX TABLETS CONTAINING AMBROXOL HYDROCHLORIDE

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
Soma Vinisha*, Sajida Akhtari Begum, Nikhat Tabassum, Soma Anusha
Department of pharmaceutical science,
Bharat Institution of Techology, Hyderabad, India
choti.reddy@gmail.com

ABSTRACT
Purpose:
This study aimed to formulate Ambroxol hydrochloride SR matrix tablets using xanthan gum (natural polymer) and to elucidate the release kinetics of Ambroxol hydrochloride from xanthan gum-matrices. Methods: controlled release matrix tablets of ambroxol hydrochloride, a mucolytic expectorant  were prepared by wet granulation method using xanthan gum as natural hydrophilic polymer in three different ratios (Drug : Polymer 1:1(F-1), 1:1.5(F-2), 1:2(F-3)). The prepared granules of three different formulations were evaluated for angle of repose, bulk density (BD), tapped density (TD) and compressibility index (CI), hausners ratio. The prepared tablets were tested for physical parameters like weight variation, hardness, friability, content of active ingredient and In-vitro drug release studies. Results: The results obtained were within the prescribed limits. The release of ambroxol from the matrix tablets was sustained up to 12hrs. The cumulative percentage of drug release was decrease with increase in polymer concentration. Among the three formulations F-3 gave the release profile close to the commercially available marketed sample of ambroxol Hcl (A-MS).The results indicate that the drug release from the matrix tablets followed Zero order kinetics. The dissolution data was fitted to Korsmeyer equation which is used to describe the drug release behaviour from polymeric systems. All the formulations showed diffusion co-efficient value (n) greater than 0.43 but less than 0.85 after fitting to the Korsmeyer equation. So, it indicates Non-Fickian transport mechanism. Therefore the drug release is by diffusion and erosion mechanism. Conclusion: Matrix tablets of Ambroxol Hydrochloride using xanthan gum prepared by wet granulation method were found to be good in appearance. The drug-polymer ratio was found to influence the release of drug from the formulations. Formulation F-3 i.e. (1:2 drug: polymer) exhibited the similar In-vitro drug release rates as that of the marketed sample.