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Pharmaceutical Analysis Articles


FORCED DEGRADATION AND STABILITY TESTING: STRATEGIES AND ANALYTICAL PERSPECTIVES

ABOUT AUTHORS:
U. A. Deokate, A. M. Gorde*
Dept. Of Pharmaceutical Chemistry,
Government College of Pharmacy, Hotel Vedant Road, Osmanpura,
Aurangabad, Maharashtra, India 431005
gordeanjali@gmail.com

ABSTRACT:
This review discusses the regulatory aspects of forced degradation and methodology aspects for degradant investigations. It also focuses on the prediction of degradation products and pathways and development of stability indicating assay method. While reviewing the analytical perspectives various conventional and hyphenated techniques for degradant separation and characterization are described in detail.


ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF FIRST ORDER DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF AMILORIDE AND TORSEMIDE IN THEIR COMBINED PHARMACEUTICAL DOSAGE FORM

About Authors:
Krutika J. Bhalodiya*, Nehal Ghelani, Darshan Madiya, Shital Faldu
Department of Quality Assurance
Smt. R. D. Gardi B. Pharmacy College, Rajkot
bhalodiyakruti1@gmail.com

Abstract
UV Spectrophotometric method has been developed for simultaneous estimation of Amiloride (AML) and Torsemide (TSM) in bulk drug and in their combined dosage form by first order derivative. This method utilizes methanol as a solvent and λmax of Amiloride and Torsemide selected for analysis was found to be 248 nm(at ZCP of TSM) and 308 nm (at ZCP of AML) respectively. Linearity was observed in the concentration range of 4-14μg/ml for AML (r2 = 0.999) and 4-20 μg/ml for TSM  (r2 = 0.998). The accuracy and precision were determined and found to comply with ICH guidelines. This method showed good reproducibility and recovery with % RSD in the desired range. The proposed methods can be successfully applied for the routine analysis of both the drugs. This method was simple, rapid, accurate, and sensitive.


FLASH CHROMATOGRAPHY: AREA & APPLICATIONS

ABOUT AUTHORS:
Ayare P*, Khanvilkar V, Chalak N
Department of Quality Assurance,
Bharati Vidyapeeth’s College of Pharmacy, Navi Mumbai
abprachi@gmail.com

ABSTRACT
Most of the sophisticated analytical techniques like NMR, MS and FT-IR need sample in the extreme pure form which is usually achieved by Preparative column chromatography but it is time consuming. From past few decades, technique called as Flash chromatography is developed which is a modification of Preparative column chromatography. This is an air pressure driven technique comprising of medium and short column chromatography, optimised for rapid separation of organic compounds. Modern flash chromatographic system consists of pre-packed plastic cartridges wherein solvent is pumped through the cartridges to achieve separation. These systems are also linked with detectors and fraction collectors which can even be automated. It is a simple, fast and economic approach to preparative liquid chromatography for purification of chemical species.This review highlights the principle involved, instrumentation, general procedure and advancement in Flash chromatography along with its applications.


ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF FIRST ORDER DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL AND PROPYPHENAZONE IN THEIR COMBINED PHARMACEUTICAL DOSAGE FORM

About Authors:
Mehul Kakdiya*, Avinash Nagapara, Darshan Madiya, Shital Faldu
Department of Quality Assurance,
Smt. R. D. Gardi B. Pharmacy College, Rajkot, Gujarat, India
*kakdiyamehul@gmail.com

Abstract
A simple, precise, accurate and reproducible spectrophotometric method has been developed for  simultaneous estimation of Paracetamol (PCM) and Propyphenazone (PP) by employing first order derivative zero crossing method in Methanol. The first order derivative absorption at 249 nm (zero cross point of Paracetamol) was used for quantification of Propyphenazone and 274 nm (zero cross point of Propyphenazone) for quantification of Paracetamol. The linearity was established over the concentration range of 1-12 µg/ml and 5-24 µg/ml for Paracetamol and Propyphenazone with correlation coefficient r2 0.999 and 0.996, respectively. The mean % recoveries were found to be in the range of 100.48-102.119% and 100.448-102.713 % for Paracetamol and Propyphenazone, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of Paracetamol and Propyphenazone in their combined pharmaceutical dosage form.


Q-ABSORBANCE RATIO SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PARACETAMOL AND PROPYPHENAZONE IN THEIR COMBINED PHARMACEUTICAL DOSAGE FORM

About Authors:
1Mehul Kakdiya*, 2Viral Kakdiya, 1Darshan Madiya
1Department of Quality Assurance,
Smt. R. D. Gardi B. Pharmacy College, Rajkot
2Pacific college of pharmacy, Udaipur, Rajasthan
*kakdiyamehul@gmail.com

Abstract
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical Q-absorbance ratio method for the simultaneous determination of Paracetamol and Propyphenazone in their combined pharmaceutical dosage form. Absorbance ratio method uses the ratio of absorbance at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components.  Paracetamol and Propyphenazone show an isoabsorptive point at 264 nm in methanol.The second wavelength used is 249 nm, which is the λ-max of Paracetamol in methanol. The linearity was obtained in the concentration range of 1-12 μg/ml for Paracetamol and 5-24 μg/ml for Propyphenazone. The concentrations of the drugs were determined by using ratio of absorbance at isoabsorptive point and at the λ-max of Paracetamol. The method was successfully applied to pharmaceutical dosage form because of no interference. The results of analysis have been validated by recovery studies.


NEW SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF LUMEFANTRINE

ABOUT AUTHORS:
*1Baokar Shrikrishna, 1Annadate Amol, 2Undare Santosh
1Department of Pharmaceutical Chemistry, SVPM’s College of Pharmacy, Malegaon (Bk II), Tal-Baramati, Dist- Pune, Maharashtra, India
2P.G. Department of chemistry, Balbhim arts, science and commerce college, Beed
krishnabaokar@gmail.com

ABSTRACT
A Simple, sensitive, specific, spectrophotometric method has been developed for the detection of Lumefantrine in pure and Pharmaceutical formulations. The optimum condition for the analysis of the drug was established. Lumefantrine shows maximum absorption at 228 nm and obeyed beers law in the concentration range 10 to 50 µg/ml.

The correlation coefficient was found to be 0.999 and slope of line was found to be 0.0635. The percent S.D. for intra assay precision of the method was found to be 1.85% whereas Inter assay precision was found to be 0.44%. The sample solution was stable up to 24 hours. The assay results were found to be in good agreement with label claim.

The proposed method was simple sensitive, precise, quick and useful for routine quality control.


Q-ABSORBANCE RATIO SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND CANDESARTAN CILEXETIL IN SYNTHETIC MIXTURE

ABOUT AUTHORS:
Kotecha B*, Pambhar M, Zala G, Faldu S
Department of Quality assurance, Smt. R.D. Gardi B.Pharmacy College,
Nyara, Rajkot-360 110, Gujarat, India
kotecha_bijal29@yahoo.com

ABSTRACTS:
The present manuscript describe simple, sensitive, rapid, accurate, precise and economical q-absorbance method for the simultaneous determination of Amlodipine besylate and Candesartan cilexetil in synthetic mixture. Absorbance ratio method uses the ratio of absorbance at two selected wavelengths, one which is an Isoabsorptive point at 242 nm in Methanol. The second wavelength is used 255 nm, which is λmax of Candesartan cilexetil in Methanol. The linearity was obtained in the concentration range of 5-25 μg/mlof both drugs. The concentration of drugs was determined by using ratio of absorbance at isoabsorptive point and at the λ-max of Candesartan cilexetil. The method was successfully applied to pharmaceutical dosage form because of no interference. The result of analysis has been validated by recovery studies.


UV-VISIBLE SPECTROSCOPY: AN OVERVIEW

ABOUT AUTHORS:
Kambham Venkateswarlu1*, N.Devanna2, Haritha Arikeri3, M.Shahinaz Farihath4
1,4M.Pharm Scholar, Department Of Pharmaceutics,
2Director Of JNTUA-Otri,
3M.Pharm Scholar, Department Of Pharmaceutical Analysis
JNTUA-Oil Technological Research Institute,
Beside Collector Office, Anantapur, Anantapur District, Andhra Pradesh, India. Pin Code: 515001
*k.v.reddy9441701016@gmail.com

ABSTRACT:
Spectroscopy is often used in physical and analytical chemistry for the identification of substances through the spectrum emitted from or absorbed by them. Spectroscopy is also heavily used in astronomy and remote sensing. Most large telescopes have spectrometers, which are used either to measure the chemical composition and physical properties of astronomical objects or to measure their velocities from the Doppler Shift of their spectral lines.
UV-Visible spectroscopy is a form of Absorption spectroscopy. Absorption spectroscopy in the UV-Visible region is to be one of the oldest and most frequently employed technique in pharmaceutical analysis for qualitative, quantitative and structural analysis of a substance in solution. The substance is analyzed by studying the spectrum produced by it due to absorption of certain wavelengths of UV-Visible light.
Spectroscopically, visible light behaves in a similar way as UV light. Hence, the techniques of UV spectroscopy and Visible spectroscopy are studied together.


A COMPLETE REVIEW ON NUCLEAR MAGNETIC RESONANCE (NMR)

ABOUT AUTHORS:
*Md. JahaSultana, P. Vijaya Sri, G.Alekhya, S.VenkateswaraRao, Chaitanya PrasadMeher
Department Of Pharmaceutical Analysis
Vijaya Institute of Pharmaceutical Sciences For Women
Enikepadu, Vijayawada-521108, Andhra Pradesh, India
sohnivya786@gmail.com

ABSTRACT:
Over a past decades, nuclear magnetic resonance (NMR) has progressed rapidly in improvement of experimental method and development of novel approaches. NMR is a research technique that exploits the magnetic properties of certain atomic nuclei. Of all the spectroscopy methods, NMR is the only one of which a complete analysis and interpretation of the entire spectrum is normally excepted. Although larger amounts of samples are needed when compared with mass spectroscopy, NMR is non-destructive and with modern instruments good data may be obtained from samples weighing less than a milligram. In this review a brief introduction to the NMR is given which includes instrumentation, parameters influencing NMR and applications.


A CRITICAL REVIEW ON PHARMACEUTICAL ANALYSIS OF NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY (HETCOR SPECTROSCOPY)

About Authors:
Reshma. K*, M.Muthukumaran*, B.krishnamoorthy, Amreen Nishat
Montessori Siva sivani Institute of Science&Technology- College Of
Pharmacy
Vijayawada, Andhrapradesh-521 230
reshmakaaja@gmail.com

Abstract
Nuclear magnetic resonance (NMR) has progressed rapidly over the last decade as a result of improved experimental technology and development of novel approaches. NMR spectroscopy has evolved into an important technique in support of structure-based drug design. It was most useful as a technique to provide structural information regarding protein drug targets and target–ligand interactions. More recently, it has been shown that NMR may be used as an alternative method for identification of small molecule ligands that bind to protein drug targets. High throughput implementation of these experiments to screen small molecule libraries may lead to identification of potent and novel lead compounds. NMR as a probe of microscopic dynamic behaviour through relaxation and direct diffusion measurements over a wide temperature range is examined.


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