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Pharmaceutical Analysis Articles


A SIMPLE SPECTROPHOTOMETRIC ESTIMATION OF CEFTRIAXONE SODIUM IN BULK AND STERILE FORMULATION

ABOUT AUTHORS:
M. Jambulingam*, S. Ananda Thangathurai1, D. Kamalakannan1, S. Punitha1, Rincy T.R1, S. Santhi1, G. Surya1, M. Vasanthi.1, S. Josephine Subarla.1
Department of Pharmaceutical Analysis, Swamy Vivekanandha College of Pharmacy,
Elayampalayam-637205, TN, India
*jambulingam48@gmail.com

ABSTRACT:
A simple spectrometric method has been developed for the estimation of the ceftriaxone sodium in powder for injection dosage form by derivatization with p-dimethyl amino benzaldehyde. The measurement of absorbance and derivatized ceftriaxone sodium at 490.4nm. The both methods obeys Beer’s and Lambert’s law in the range of 5-25µg/ml with the correlation co-efficient of r²0.998. The colour reaction was highly stable and didn’t show any changes in absorbance up to 48hrs. The % RSD associated with all the validation parameter was less than 2, showing the accuracy of the method developed. The compliance of acceptance criteria of Q2 (R1), (R2) international conference on harmonization (2005 guidelines).


ESTIMATION OF ONDANSETRON HYDROCHLORIDE IN BULK AND FORMULATION BY SECOND ORDER DERIVATIVE AREA UNDER CURVE UV-SPECTROPHOTOMETRIC METHODS

ABOUT AUTHORS:
Jadhav Santosh1*, Kharat Rekha1, Ansari Afaque2, Tamboli Ashpak3
1Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra, India.
2Department of Pharmaceutic’s, D. S. T. S Mandal’s College Of Pharmacy Solapur, Maharashtra India.
3Department of Pharmaceutical chemistry, Sahyadri College of Pharmacy, Methwade,
Sangola-413307, Solapur, Maharashtra, India.
*jadhavsan88@gmail.com

ABSTRACT:
Simple, fast and reliable spectrophotometric methods were developed for determination of Ondansetron Hydrochloride in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Distilled Water. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 248-254nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ondansetron Hydrochloride using 2-10μg/ml (r²=0.9986) for second order Derivative Area under Curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ondansetron Hydrochloride in pharmaceutical formulations.


DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF LAMOTRIGINE AND CLOZAPINE IN SYNTHETIC MIXTURE BY ABSORPTION CORRECTION METHOD

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ABOUT AUTHORS:
Priyanka P. Atodariya*, Hasumati A. Raj, Vineet C. Jain
*Shree Dhanvantary Pharmacy Collage, Kim,
Surat, Gujarat, India
*atodariya.priyanka@yahoo.com

ABSTRACT:
The simple spectroscopic method has been developed for simultaneous estimation of Lamotrigine and Clozapine in synthetic mixture. Absorbance Correction Method involves the measurement of absorption at two wavelengths 307 nm (lmaxfor Lamotrigine) and 360 nm (lmax for Clozapine). The method was found linear between the range of 1-5 µg/ml for Lamotrigine and 6-30 µg/ml for Clozapine for method. The accuracy and precision was determined and validated statistically. Both the method showed good reproducibility and recovery with %RSD less than 1. The method was found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis for Lamotrigine and Clozapine in bulk and combined dosage form.


NEED AND APPLICATION OF ANALYTICAL METHOD DEVELOPMENT ON NEW FIXED DOSE COMBINATION OF IRBESARTAN AND ATORVASTATIN IN PHARMACEUTICAL INDUSTRY

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ABOUT AUTHORS:
Paras Virani1,2*, Parul Jain3, Hasumati Raj2, Vineet Jain2
1Research Scholar 2014, Gujarat Technological University, Gujarat
2Quality assurance department, Shree Dhanvantary Pharmacy College, Kim, Surat
3Quality assurance department, Maliba Pharmacy College, Bardoli, Surat
parasvirani@gmail.com

ABSTRACT
Analytical method is primary requirement of the pharmaceutical industry. In pharmaceutical industry various analytical methods is used like chromatography, spectroscopy method, electrochemical method, ion incorporating method, etc. Irbesartan is classified as an angiotensin II receptor type 1 antagonist. Angiotensin II receptor type 1 antagonists are widely used in treatment of diseases like hypertension, heart failure, myocardial infarction and diabetic nephropathy. Atorvastatin is the most efficacious of the currently available HMG-CoA Reductase inhibitors used in anti lipidemic and also used in atherosclerosis, stroke and cardiac risk. In recently approved new fixed dose combination of Irbesartan and atorvastatin in market of Korea so it require analytical method development which help in industry for new drug delivery system development. This review highlights the role, need and application of various analytical techniques for Irbesartan and atorvastatin combination and their corresponding analytical methods in the pharmaceutical industry.


MOLECULAR DOCKING STUDIES OF N-(2-BENZOYLPHENYL)-L-TYROSINE DERIVATIVES WITH ANTI-DIABETIC ACTIVITY OF TYPE 2 DIABETES

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ABOUT AUTHORS:
Anuradha Sharma1*, Vaibhav Walia2, Monika Gahlawat3
1Division of Pharma. Chemistry,2 Division of Pharmacology,3 Division of Pharmaceutics,
G.V.M. College of Pharmacy,
Sonepat, Haryana, India
*anusarswat@gmail.com

ABSTRACT
Type 2 diabetes is one of the major life threatening diseases worldwide. These cases are progressing at an incremental rate every year and number of research works is going on to control the disease by targeting its enzymes or proteins. In modern drug designing, molecular docking is routinely used for understanding drug receptor interaction. In the present study molecular docking were performed on a diverse set of N-(2-benzoylphenyl)-L-tyrosine derivatives that demonstrate antidiabetic activity by stimulating peroxisome proliferator activated receptor- γ. The docking program in Glide dock justifies the correlation between the experimental values and the values derived computationally. Therefore, the dock analysis performed in Glide dock suggests the importance of evaluating the prediction accuracy of scoring functions adopted in various docking program.


METHOD VALIDATION OF ANALYTICAL PROCEDURES

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ABOUT AUTHORS
Prakash Chanda Gupta
QC Executive,
National Healthcare Pvt. Ltd., Nepal
p_c_gupta@yahoo.com

ABSTRACT
After the development of an analytical procedure, it is must important to assure that the procedure will consistently produce the intended a precise result with high degree of accuracy. The method should give a specific result that may not be affected by external matters. This creates a requirement to validate the analytical procedures. The validation procedures consists of some characteristics parameters that makes the method acceptable with addition of statistical tools.


RP-HPLC METHOD FOR THE ESTIMATION OF NITAOXANIDE IN PHARMACEUTICAL FORMULATION

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ABOUT AUTHORS:
R.Meera1*, N.Swathylakshmi2, M.Sundarapandian2, P.Raja Soundara Pandian1, Madhavanmallayasamy1
1Researcher, Radianz Health Care Pvt Ltd, Madurai, Tamilnadu, India
2Department of pharmaceutical Chemistry, K.M.College of pharmacy, Uthangudi, Madurai, India
meeraharsa23@gmail.com

ABSTRACT
Objective
: A simple and precise RP-HPLC method was developed and validated for the determination of Nitaoxanide in pharmaceutical dosage forms.
Materials and Methods
: Chromatography was carried out using waters RP –C18 150×4.6 mm, 3.5 µ, pH 6.8, buffer: acetonitrile (50:50) as the mobile phase at a flow rate 1.2 ml/min. The analyze was monitored using PDA detector at 254 nm. The proposed method was found to have linearity in the concentration range of 25-150µg/ml with correlation co efficient of r2 =0.9999.
Results:
The developed method has been statistically validated and found simple and accurate. The mean recoveries obtained for Nitaoxanide were in the range 100.06-101.9%.
Conclusion:
Due to its simplicity, rapidness, high precision and accuracy of the proposed method it may be used for determining Nitaoxanide in bulk and dosage forms.


SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF NITAOXANIDE IN BULK DRUG AND ITS PHARMACEUTICAL FORMULATION

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ABOUT AUTHORS:
R.Meera1*, N.Swathylakshmi2, M.Sundarapandian2, P.Raja Soundara Pandian1, Madhavanmallayasamy1
1Researcher, Radianz Health Care Pvt Ltd, Madurai, Tamilnadu, India
2Department of pharmaceutical chemistry, K.M.College of pharmacy, Uthangudi, Madurai, India
meeraharsa23@gmail.com

ABSTRACT
Objectives
:
A simple spectrophotometric method was developed and validated for the determination of Nitaoxanide in pharmaceutical dosage forms. Two visible spectrophotometric methods have been described for the assay of Nitaoxanide bulk form or dosage forms.
Methods:
Method A is based on the formation of Schiff’s base and it was condensed with 4 hydroxybenzaldehyde. Method B is based on diazotization and coupling method with phluroglucinol. The methods are done in UV Visible spectrophotometric method having maximum absorbance at 460 nm.
Results:
Regression analysis of Beers law plots showed good concentration range of 10-50µg/ml for method A and B and gives reproducible results.
Conclusion
: Due to its simplicity of the method it may be used for determining Nitaoxanide in bulk and dosage forms.


A REVIEW ON ANALYTICAL METHODS FOR DETERMINATION OF LEVOSULPIRIDE IN PHARMACEUTICAL DOSAGE FORMS AND BIOLOGICAL SAMPLE

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ABOUT AUTHORS:
Monika A. Rana*, Hasumati A. Raj
Department of Quality Assurance
Shree Dhanvantary College of Pharmacy,
Kim, Gujarat, India
monika92rana@gmail.com

ABSTRACT
Levosulpiride is an atypical antipsychotic agent. Levosulpiride is the levo enantiomer of sulpiride. It is a substitute benzamide which is meant to be used for several indications: depression, psychosis, somatoform disorders, emesis anddyspepsia. It blocks the presynaptic dopaminergic D2 receptor. Chemically it is N-[[(2S)-1-Ethylpyrrolidin-2-yl] methyl]-2-methoxy-5 sulfamoylbenzamide. several method such as HPLC in human plasma, area under curve, stability by RP-HPLC is done. The parent drug is given in a dose of 400-1800 mg orally. According to literature survey study of impurity profiling of LIVOSULPIRIDE in presence of intermediate has not been reported.


A REVIEW OF ANALYTICAL METHODS FOR DETERMINATION BROMHEXINE HYDROCHLORIDE IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES

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ABOUT AUTHORS:
Meera V. Lad1*, Vineet Jain2, Hasumati Raj1
1Department of Quality Assurance,
2Department of Pharmacognosy,
Shree Dhanvantary Pharmacy College, Kim, Gujarat
meeralad235@gmail.com

ABSTRACT:
Bromhexine HCl (BRH)is a mucolytic agent used in the treatment of respiratory disorders associated with viscid or excessive mucus, chemically named 2-amino-3,5-dibromo-N-cyclohexyl-N-methyl benzenemethanamine hydrochloride. According to IUPAC it is 2,4-dibromo-6-[[cyclohexyl(methyl)amino]methyl] aniline hydrochloride. Because of its physiological importance, the drug has been quantified by exploiting its chemical  and physical properties. Bromhexine is a weak base and its precipitate out at pH value above 6. Bromhexine is a synthetic benzyl amine derivative ofvasicine. The different analytical methods used to quantify the drug as a single active pharmaceutical ingredient include flow injection analysis with ionselectiveelectrodes, inductively coupled plasma mass spectrometry, electrokinetic chromatography, electrochemical oxidation at the glassy carbon electrode, liquid chromatography, liquid gas chromatography, GC with mass detection, and voltammetry. The drug has also been quantified in its combined formulations using HPLC, direct and derivative UV spectrophotometry.


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