Indukaka Ipcowala College of Pharmacy

DESIGN, DEVELOPMENT AND OPTIMIZATION OF OLMESARTAN MEDOXOMIL LIQUISOLID TABLETS USING CENTRAL COMPOSITE DESIGN

ABOUT AUTHORS:
Swati k. Nagar*1, Dr. Harsha V. Patel1, Dr.Vishnu A. Patel2, Vinod V. Siju3
1Indukaka Ipcowala College of pharmacy, New V.V.Nagar
2ARCP, Vallabh vidhyanagar
3Anand Pharmacy College, Anand.
*swati.nagar28@gmail.com

ABSTRACT-
The aim of the present study was to investigate the applicability of liquisolid technique in improving the dissolution properties and solubility of olmesartan medoxomil (OLM) in a solid dosage form. This study was designed to optimize and evaluate the effects of different formulation variables: ratio of carrier to coating material (X1) and drug concentration (X2)  on angle of repose (Y1), hardness(Y2), saturation solubility study (Y3) and cumulative percentage release at 10 min (CPR 10min)(Y4) of formulation using five  level two factor central composite design. The quadratic model generated by the design is of the form: Y = A0 + A1X1 + A2X2 + A3X1X2 + A4X12 + A5X22 + E, where Y is the measured response associated with each factor level combination. Contour and response surface plots were depicted based on the equation given by the model. The optimized formula yields observed values close to the predicted values. The liquisolid tablets were formulated with liquid vehicles, poly ethylene glycol 400 (PEG400) at five drug concentrations, 10% w/w, 15% w/w, 20% w/w, 25% w/w and 30% w/w. Avicel PH102 was used as a carrier material, Aerosil 200 as a coating material and cross carmellose sodium as a disintegrant.In-vitro drug dissolution profiles of the liquisolid formulations were studied and compared with conventional formulation (olmezest), in simulated intestinal fluid (pH 6.8). The drug release rates of LS compacts were higher as compared to directly compressed tablets, which show significant benefit of LS in increasing wetting properties and surface area of drug available for dissolution. From this study it concludes that the LS technique is a promising alternative for improvement of dissolution property.

Automated analysis

About Authors:
Patelia emanual michael*, Keyur B Ahir
Department of Pharmaceutical Chemistry and Analysis,
Indukaka Ipcowala College of Pharmacy,
New Vallabh Vidyanagar – 388121, Gujarat, India.
*ricky.emanual@gmail.com

Abstract:
Ethane, cyclohexane, SO2, ethylene and other gaseous components. Liquid solutions also can be monitored by the analyzers. Near IR measurements using tungsten filaments lamps as sources are often used to monitor water concentrations. IR reflectance can be used to monitor water concentration in some solids, such as in paper. If moisture is present some of the incident radiation is observed and less is reflected at the wavelengths characteristics of water.

ESTIMATION OF DRONEDARONE HYDROCHLORIDE BY SPECTROPHOTOMETRIC METHOD IN PHARMACEUTICAL FORMULATION

About Authors:
Kashyap K. Bhatt, Emanual Michael Patelia*, Ishani Amin
Department of Pharmaceutical Chemistry and Analysis,
Indukaka Ipcowala College of Pharmacy,
New Vallabh Vidyanagar – 388121, Gujarat, India.
*ricky.emanual@gmail.com

Summary
The UV- spectrophotometric method was developed for estimation of DRO in its dosage form. UV- spectrophotometric method was developed using methanol as solvent. Detection Wavelength was found to be 289nm. Detection wavelength was 289nm. In UV- spectrophotometric linear range was found to be 6 – 16 μg/ml & mean recoveries were found to be 99.83-100.04%. This spectroscopic method is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined tablet dosage forms.


PEPTIDE MAPPING

About Authors:
Gautambhai, Emanual Michael Patelia*, Arpit Shah
Department of Pharmaceutical Chemistry and Analysis,
Indukaka Ipcowala College of Pharmacy,
New Vallabh Vidyanagar – 388121, Gujarat, India.
*ricky.emanual@gmail.com

General Concept:

  • A fragmentation pattern generated by digestion of a particular protein with proteolytic enzymes of known specificity.
  • Used in protein identification.
  • Proteases will produce fragments of a characteristic size from a protein and this can be used as a test for the identity or otherwise of two similar sized proteins.
  • Peptide mapping involves controlled cleavage of a pure protein with small amounts of a pure protease to generate peptides of characteristic, reproducible sizes.

SIMULTANEOUS ESTIMATION OF TRAMADOL HCL, PARACETAMOL AND DOMPERIDONE IN PHARMACEUTICAL FORMULATION BY RP-HPLC METHOD

About Authors:
Keyur B.ahir, Emanual M. Patelia*, Falgun A.Mehta
Department of Pharmaceutical Chemistry and Analysis,
Indukaka Ipcowala College of Pharmacy,
New Vallabh Vidyanagar – 388121, Gujarat, India.
*ricky.emanual@gmail.com

Abstract:
A simple, precise, rapid, selective, and economic reversed phase high-performance liquid chromatography (RP-HPLC) method has been established for simultaneous analysis of A Phenomenex C18 (250´4.6 mm i.d) chromatographic column equilibrated with mobile phase 0.02M Potassium dihydrogen o-phosphate/acetonitrile (55/45, v/v) adjusted to pH 6.5 with Triehtylamine (1% v/v) was used. Mobile phase flow rate was maintained at 1 ml/min and effluents were monitored at 278 nm. The sample was injected using a 20 ml fixed loop, and the total run time was 10 min. Experimental conditions such as pH of mobile phase, column saturation time, selection of wavelength, etc. were critically studied and the optimum conditions were selected. The retention time for PCM, DMP and TMD were 3.76 min, 5.18 min and 4.28 min, respectively. The calibration curve for DMP, PCM and TMD was found to be linear in the range of 0.2 - 1 mg/ml, 6.5 – 32.5 µg/ml and 0.75 – 3.75 mg /ml with a correlation coefficient of 0.9998, 0.9976 and 0.9974. The detection limits for PCM, DMP and TMD were 20 ng/ml, 1.06 ng/ml and 2 ng/ml, respectively, while quantitation limits were 60 ng/ml, 3.23 ng/ml and 6 ng/ml, respectively. This HPLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined tablet dosage forms.

Simultaneous Estimation of Tramadol HCl, Paracetamol and Domperidone in Pharmaceutical Formulation by Thin-Layer Chromatographic-Densitometric method

About Authors:
Keyur B.ahir, Emanual M. Patelia*, Falgun A.Mehta
Department of Pharmaceutical Chemistry and Analysis,
Indukaka Ipcowala College of Pharmacy,
New Vallabh Vidyanagar – 388121, Gujarat, India
*ricky.emanual@gmail.com

Abstract:
A simple, precise, rapid, selective, and economic high-performance-thin-layer chromatography (HPTLC) method has been established for simultaneous analysis of Domperidone (DMP), Paracetamol (PCM) and Tramadol Hcl (TMD) in tablet dosage forms. The chromatographic separations were performed on precoated silica gel 60254 plates with toluene-ethylacetate-butanol-ammonia 5:4:1:0.2(v/v) as mobile phase. The plates were developed in a 7.0 cm at ambient temperature. The developed plates were scanned and quantified at their single wavelength of maximum absorption at approximately 278 nm for DMP and PCM, respectively. Experimental conditions such as chamber size, chamber saturation time, migration of solvent front, slit width, etc. were critically studied and the optimum conditions were selected. The drugs were satisfactorily resolved with Rf 0.18 ± 0.02 for DMP, Rf 0.25 ± 0.02 for PCM and for TMD Rf 0.50 ± 0.02. The method was validated for linearity, accuracy, precision, and specificity. The calibration plot was linear between 100-600 ng / band for DMP, 3250-19500 ng / band based for PCM and 375-2250 ng / band based for TMD. The limits of detection and quantification for DMP were 9.95 and 30.16ng / band, respectively; for PCM they were 64.30 ng and 194.87 ng / band and for TMD 5.51 and 16.70/ band. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined tablet dosage forms.