Pharmaceutical Analysis

About Authors:
Bhawana Kapoor^*1, Vishnukant Rai², Sonu Sharma^3
1Seth G.L. Bihani S.D. College of Technical Education, Sriganganagar, Rajasthan, India
²Shri Ramnath Singh College of Pharmacy, Gormi, Bhind, M.P., India
³NIMS Institute of Pharmacy, Jaipur, Rajasthan, India

ABSTRACT
Chromatography, although primarily a separation technique, is mostly employed in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. The reasons for this include the simplicity, versatility, and scope of the reversed-phase method as it is able to handle compounds of a diverse polarity and molecular mass. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development.

About Authors:
Kalpesh N. Patel¹*, Jayvadan K. Patel^2
1 Research Scholar, Jodhpur National University, Jodhpur,
Rajshathan, India
² Department of Pharmaceutical Technology,
Nootan Pharmacy College,
Visnagar (GUJARAT), India.

Abstract
Two simple spectrophotometric methods have been developed for simultaneous estimation of pyrimethamine and sulphadoxine from tablet dosage form. Method-I simultaneous equation method involves the measurement of absorbances at two wavelengths 215 nm (λmax of pyrimethamine) and 254 nm (λmax of sulphadoxine) in methanol and Method-II first order derivative spectroscopic method involves the measurement of absorbances at two wavelengths 250 nm (λmax of pyrimethamine) and 220.5 nm (λmax of sulphadoxine); The linearity lies between 5-30 µg/ml for both pyrimethamine and sulphadoxine for all the two methods. The accuracy and precision of the methods were determined and validated stastically. All the methods showed good reproducibility and recovery with % RSD less than 2. All method were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of pyrimethamine and sulphadoxine in bulk and combined dosage form.

The college "Saraswati School of Pharmacy" is located at- Ranela, Mahesana. This college runs B. Pharm course in the college campus. The college is run by Shree B. M. Patel Education Trust. This is one of the largest educational campus running professional institutions in the University, to which it is affiliated with. It offers educational course.

Optimal environment to natural, personal and professional development leading to the making of responsible citizens. The day begins in the holy and spiritual environment with a prayer. Complete discipline, unfitness and the unity brought to be attained thought a dress code for staff members and students.

The National Institute of Pharmaceutical Education & Research (NIPER) is an Institute of National Importance by an Act of Parliament under the aegis of Department of Pharmaceuticals, Ministry of Chemicals & Fertilizers, Government of India. The institute is located at S.A.S. Nagar, Punjab adjoining Chandigarh the City Beautiful.

SAL Institute of Pharmacy (SALIP) situated in SAL Education campus was established in June-2009 under the aegis of Adarsh Foundation with a view of educating and training young talented students of Gujarat at the field of Pharmacy. It is a self-financed institute approved by All India Council for Technical Education (AICTE), New Delhi, the Government of Gujarat and afileted by Gujarat Technological University.

The Mother Theresa Post Graduate and Research Institute of Health Sciences was established by the Government of Puducherry to develop the requirements of health care personnel by providing health care educational courses for the Union Territory of Puducherry.