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Spin-spin splitting and factors influencing coupling constant

Q.3.(a)Spin-spin splitting and factors influencing coupling constant

Modern analytical techniques - Solved Paper 2008-09
Discuss ELISA assay for antibody by indirect methods and antigen by sandwich method

Q.2. (c) Discuss ELISA assay for antibody by indirect methods and antigen by sandwich method. Enzyme Linked ImmunosorbentAssay (ELISA)

Ans.2. (c)The steps of the general, "indirect," ELISA for determining serum antibodyconcentrations are:

How would you identify various functional groups by IR spectroscopy provide supporting examples.

Q.2. (b)How would you identify various functional groups by IR spectroscopy provide supporting examples.

Ans.2. (b)Identify various functional groups by IR spectroscopy:

Draw line diagrammes and discuss mode of operation of dispersive and interferomatric IR instruments with their merits & demerits

Q.2. (a) Draw line diagrammes and discuss mode of operation of dispersive and interferomatric IR instruments with their merits & demerits. 

Ans.2. (a) Mode of operation of dispersive IR instrument

Write a note on ATR and its application in specific cases.

Q.1. (c) Write a note on ATR and its application in specific cases.

Ans.1. (c)ATR: Internal reflection spectroscopy is a technique for obtaining IR spectra of samples that are difficult to deal with, such as solids of limited solubility, films, threads,pastes, adhesives, and powders. 

Cotton effect curves and octant rule

Q.1.(b) Cotton effect curves and octant rule, How would you relate stereo chemical features of a compound with them?

Ans.1. (b) Cotton Effect Curve

Define and exemplify chromophores

Q.1. (a)Define and exemplify chromophores. Discuss their interaction with UV- visible radiation and illustrate their use in structural analysis of organic compounds.

Ans.1. (a) CHROMOPHORE: The term chromophore was previously used to denote a functional group of some other structural feature of which gives a color to compound. For example- Nitro group is a chromophore because its presence in a compound gives yellow color to the compound. But these days the term chromophore is used in a much broader sense which may be defined as “any group which exhibit absorption of electromagnetic radiation in a visible or ultra-visible region “It may or may not impart any color to the compound. Some of the important chromophores are: ethylene, acetylene, carbonyls, acids, esters and nitrile groups etc. A carbonyl group is an important chromophore, although the absorption of light by an isolated group does not give rise to any colour in the ultra-violet spectroscopy.

Modern analytical techniques - Solved Paper 2007-08
Explain Electrophoresis, its principle and factors governing it

Q.5. (c) Explain Electrophoresis, its principle and factors governing it
Ans.5.(c) Electrophoresis: is a technique used to separate and sometimes purify macromolecules - especially proteins and nucleic acids - that differ in size, charge or conformation. As such, it is one of the most widely-used techniques in biochemistry and molecular biology.

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