Pharma Analysis

Draw line diagrammes and discuss mode of operation of dispersive and interferomatric IR instruments with their merits & demerits

Q.2. (a) Draw line diagrammes and discuss mode of operation of dispersive and interferomatric IR instruments with their merits & demerits. 

Ans.2. (a) Mode of operation of dispersive IR instrument

Define and exemplify chromophores

Q.1. (a)Define and exemplify chromophores. Discuss their interaction with UV- visible radiation and illustrate their use in structural analysis of organic compounds.

Ans.1. (a) CHROMOPHORE: The term chromophore was previously used to denote a functional group of some other structural feature of which gives a color to compound. For example- Nitro group is a chromophore because its presence in a compound gives yellow color to the compound. But these days the term chromophore is used in a much broader sense which may be defined as “any group which exhibit absorption of electromagnetic radiation in a visible or ultra-visible region “It may or may not impart any color to the compound. Some of the important chromophores are: ethylene, acetylene, carbonyls, acids, esters and nitrile groups etc. A carbonyl group is an important chromophore, although the absorption of light by an isolated group does not give rise to any colour in the ultra-violet spectroscopy.

Explain Electrophoresis, its principle and factors governing it

Q.5. (c) Explain Electrophoresis, its principle and factors governing it
Ans.5.(c) Electrophoresis: is a technique used to separate and sometimes purify macromolecules - especially proteins and nucleic acids - that differ in size, charge or conformation. As such, it is one of the most widely-used techniques in biochemistry and molecular biology.

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