PharmaTutor (July- 2014)
ISSN: 2347 - 7881
Received On: 30/04/2014; Accepted On: 07/05/2014; Published On: 01/07/2014
AUTHORS: R.A Tamboli, V.C. Chauhan, M.M. Pathan, S.K. Tirgar, D.A. Shah, R.R. Parmar
APMC College of Pharmaceutical Education and Research,
Motipura, Himmatngar, Gujarat, India
A specific, accurate, precise and reproducible RP-HPLC method has been developed and subsequently validated for the simultaneous determination of Ilaprazole and Domperidone in pharmaceutical dosage form. The proposed HPLC method utilizes hypersil (Thermo scientific) C18 column (250 mm × 4.6 mm id, 5 μm particle size), and mobile phase consisting of methanol:phosphate buffer (40:60) and pH adjusted to 4.0 with 0.1M glacial acetic acid at a flow rate of 1.0 mL/min. Quantitation was achieved with UV detection at 230 nm based on peak area with linear calibration curves at concentration ranges 5-15 μg/ml for Ilaprazole and 15-45 μg/ml for Domperidone. The retention time of Ilaprazole and Domperidone were found to be 3.433 min and 5.860 min respectively. The method was validated in terms of accuracy, precision, linearity, limits of detection, limits of quantitation and robustness. This method has been successively applied to marketed formulation and no interference from the formulation excipients was found.
How to cite this article: RA Tamboli, VC Chauhan, MM Pathan, SK Tirgar, DA Shah, RR Parmar; Development and Validation of RP-HPLC Method for Simultaneous Estimation of Ilaprazole and Domperidone in Pharmaceutical Dosage Form; PharmaTutor; 2014; 2(7); 149-156