RP-HPLC Method for the Estimation of Nitaoxanide in Pharmaceutical Formulation
PharmaTutor (December- 2014)
ISSN: 2347 - 7881
(Volume 2, Issue 12)
Received On: 30/09/2014; Accepted On: 15/11/2014; Published On: 01/12/2014
AUTHORS: R.Meera1*, N.Swathylakshmi2, M.Sundarapandian2, P.Raja Soundara Pandian1, Madhavanmallayasamy1
1Researcher, Radianz Health Care Pvt Ltd, Madurai, Tamilnadu, India
2Department of pharmaceutical Chemistry, K.M.College of pharmacy, Uthangudi, Madurai, India
Objective: A simple and precise RP-HPLC method was developed and validated for the determination of Nitaoxanide in pharmaceutical dosage forms.
Materials and Methods: Chromatography was carried out using waters RP –C18 150×4.6 mm, 3.5 µ, pH 6.8, buffer: acetonitrile (50:50) as the mobile phase at a flow rate 1.2 ml/min. The analyze was monitored using PDA detector at 254 nm. The proposed method was found to have linearity in the concentration range of 25-150µg/ml with correlation co efficient of r2 =0.9999.
Results: The developed method has been statistically validated and found simple and accurate. The mean recoveries obtained for Nitaoxanide were in the range 100.06-101.9%.
Conclusion: Due to its simplicity, rapidness, high precision and accuracy of the proposed method it may be used for determining Nitaoxanide in bulk and dosage forms.
How to cite this article: R Meera, N Swathylakshmi, MS Pandian, PRS Pandian, M Mallayasamy; RP-HPLC Method for the Estimation of Nitaoxanide in Pharmaceutical Formulation; PharmaTutor; 2014; 2(12); 145-149