Stress Induced Method Development and Validation of Olmesartan in Bulk and Pharmaceutical Dosage Form by UV Spectrometric Method

About Authors: 1Arun Kumar Dash*, 2Amitesh Palo,
1. Royal College of Pharmacy and Health Sciences, Berhampur.
2. College of Pharmaceutical Sciences, Mohuda, Berhampur

A simple method for the estimation of Olmesartan in bulk and pharmaceutical dosage forms has been developed. Methanol was chosen as the solvent system. The λmax was found to be 257nm and all absorbance values were carried out at 257nm. The responses were linear in the range of 5-35µg/ml. The regression equation of the calibration graph and correlation coefficient were found to be y = 0.049x + 0.007 and 0.999 respectively. The %RSD values for both intraday and interday precision were less than 1%. The recovery of the drug from the sample was ranged between 99.36 and 100.49%. The proposed method was validated for precision, accuracy, intraday, interday assay, robustness and ruggedness. Commercial tablets containing 20mg and 40mg of Olmesartan were analyzed by the proposed method and the results were well within the claimed limits. Furthermore stability studies of Olmesartan were carried out under acidic, alkaline, hydrolytic and photolytic conditions as per SIAM (Stability Indicating Assay Methods).

Synthesis and biological evaluation of newer analogues of 2,5-disubstituted 1,3,4-oxadiazole as antioxidant and anticonvulsant agents

About Authors: Arun Dureja*, Kalpana Divekar and M.S.Sandhyavali
Department of Pharmaceutical Chemistry,
Dayananda Sagar College of Pharmacy,
Kumaraswamy Layout,

A series of 3-(4-acetyl-5H-5-phenyl-4,5-dihydro-1,3,4-oxadiazol-2-yl)- 2H-chromen-2-one derivatives were synthesized and evaluated for their anticonvulsant and antioxidant activities. Initially Ethyl-2-oxo-2H-chromene-3-carboxylate was prepared by reacting Salicylaldehyde with Diethyl malonate in presence of piperidine. This compound was treated with hydrazine hydrate to give aromatic hydrazides. Those hydrazides were refluxed with different aldehydes to form arylidines, which were then treated with acetic anhydride to form 3-(4-acetyl-5H-5-phenyl-4,5-dihydro-1,3,4-oxadiazol-2-yl)- 2H-chromen-2-one derivatives. The structures of synthesized compounds were characterized and confirmed by IR and 1HNMR and then screened for antioxidant activity by DPPH reduction method and anticonvulsant activity by MES method. The investigation revealed that out of eight newly synthesized derivatives five were found to possess significant anticonvulsant activity but none of the derivative was found with antioxidant activity.


About Authors: Deepak Sahu
M.Pharm, Geetanjali Institute of Pharmacy
Udaipur, Rajasthan

A simple, accurate, low cost and specific HPTLC method for estimation of quetiapine fumarate in tablet has been developed. It was performed on Silica gel G60 F254 aluminium foil using acetonitrile : chloroform in the ratio of 1:9 as mobile phase. The mobile phase having chamber was saturated for 20 minutes at room temperature. The Rf value of quetiapine fumarate was found to be 0.45. The plate was scanned and quantified at 242 nm. The calibration curve response was observed between 5-25 μg. The linear regression data showed good linear relationship of r = 0.999. The percent recovery was found to be 100.0 ± 0.01.The developed method was validated for its accuracy and precision with suitable parameters.


About Author
Sayani Chakrabarati,
M.Pharm in Pharmaceutical Chemistry
SIPS, Jharpokharia

In pharmaceutical industries, many unprecedented challenges have to be faced regarding cost, time, labour, tough competition by other companies etc. To overcome these problems & curve a significant niche in global market, a new approach has been brought named lean sigma. This concept is used to make work BETTER & FASTER. Many companies have embarked on major improvement programs, including internal re-organization, greater externalization to embrace the biotech culture & capture innovation, & the movement of investments to lower cost countries. The Lean Sigma is a very positive approach in such third-world countries like India. This approach will help to beat the recession after effects very soon with appreciable efficiency.


About Authors: NEHA BATRA, S K Dubey

Ageing or aging is the accumulation of changes in an organism or object over time. Ageing in humans refers to a multidimensional process of physical, psychological, and social changes, the things you do this year can halt and even reverse many of health and cognitive issues faced by those over age 50. You could be in better shape, have fewer aches and pains AND have more energy than you did 10 years ago. Anti aging is possible. The body requires two vital materials- energy and raw material for anti-aging. There are many food and herbal treatment available in market for anti aging.


About Authors: HONEY JAIN, Mr Ranjan Bairwa

Even with improvements in health care, many Boomers are finding themselves overwhelmed with the stresses of modern life. Luckily, there are natural ways to relieve stress, including daily exercise, meditation, deep-breathing techniques and the use of time-honored herbal supplements.‘Stress response’ are many and varied-marriage, divorce, death of a loved one, new job, move, etc.-any intense emotion such as fear or anger, fatigue, physical injury, surgery, temperature extremes, noise, crowding, illness.
There is a definite relationship between stress and illness, although the exact nature of this relationship is not yet fully understood. The more frequent and severe the life changes, the more likely we are to become ill.Combination of key traditional anti-stress herbs may help settle down your world and step up your energy levels to dynamic new heights.

Preparation and Evaluation Antioxidant Activity of Mixed Herbal Hair Oil Formulation

About Authors: Akram Ahmad(1)*,Vikas Kumar(1) ,G.P.Mohanta(2) and Hasna Ali(2).
1-Christian school of pharmacy, FHMSIAMS (SHIATS) Allahabad (UP)-211007.
2-Department of pharmacy practice,Annamalai University,Chidambaram (T.N)-608002.

Antioxidants are helpful in increasing the blood circulation and thus help in hair growth as well as in the treatment a lot of diseases. Alopecia is a dermatological disorder with psychosocial implications on patients with hair loss. The Antioxidant property of plant and oil can be  utilise in hair fall .The objective of present study involves preparation of herbal hair oil using amla, brahmi, tridex, neem, shikakai and its evaluation for increase in hair growth activity. The oils with two different drug concentrations 5% & 10% were prepared using direct boiling, paste and cloth method. For determination of antioxidant activity DPPH method is used. Herbal hair oil formulation showing moderate antioxidant activity when compared with can be used as herbal hair oil for hair growth and other Free radical damage within cells has been linked to a range of disorders including cancer, arthritis, atherosclerosis, Alzheimer's disease, and diabetes.

In Vitro Dissolution Enhancement Of Felodipine

About Authors: V.P. Patel*, M.C. Gohel, R.K. Parikh
*V. P. Patel
M.Pharm, Assistant Professor, R. K. College of Pharmacy, Rajkot, Gujarat, India.
Work was carried out at: Department of pharmaceutical technology,
L. M. College of pharmacy, Ahmedabad, Gujarat, India
M. C. Gohel
Professor and Principal, Department of pharmaceutical technology,
L. M. College of pharmacy, Ahmedabad, Gujarat, India
R. K. Parikh
Professor, Department of pharmaceutical technology,
L. M. College of pharmacy, Ahmedabad, Gujarat, India

With the introduction of combinatorial chemistry and high throughput screening, the properties of new chemical entities shifted towards higher molecular weight and increasing lipophilicity that results in decreasing aqueous solubility. It is not surprising that many drug candidates have poor water solubility since the initial selection of drug candidates are based on activity alone. Other physiochemical and biopharmaceutical properties such as permeability, biopharmaceutics and metabolism are rarely considered during the selection process. The aim of this study was to increase dissolution rate of felodipine poorly soluble drugs from BCS class II by complexation process. Complexation was prepared using β cyclodextrine. Different techniques were employed for preparation of complexation with β cyclodextrin like physical mixture, cogrinding, kneading technology, solvent coevaporation.The physical properties of the prepared solid mass of felodipine was characterized by in vitro dissolution studies, UV- spectroscopy, Fourior transform infrared spectroscopy, differential scanning calorimetry (DSC) and  X-ray powder differaction spectroscopy. Additionaly, phase solubility studies were performed to support the in vitro dissolution study. The results of Fourior transform infrared spectroscopy shows the compatibility of drug with cyclodextrin, while differential scanning calorimetry (DSC) and X-ray powder differaction spectroscopy showed the confirmation of complexation of β cyclodextrin with felodipine.

Tissue Culture of Endangered Brahmi (Bacopa monnieri) (Linn) family Scrophulariaceae

About Authors: - Tiwari1 Bhrigu Narayan*, Mr. Khatri1 Pankaj, Dr. Ali2 Julfikar.
1 . Department of Pharmacognosy Gyan Vihar School of Pharmacy S.G.V. University ,Jaipur (Rajasthan)
2. Department of Biotechnology, School of Science S.G.V .University, Jaipur (Rajasthan)

Tissue culture
(often called micropropagation) is a special type of asexual propagation where a very small piece of tissue (shoot apex, leaf section, or even an individual cell) is excised (cut-out) and placed in sterile (aseptic) culture in a test tube, petri-dish or tissue culture container containing a special culture medium.Several industries and nurseries have standardized protocols for the multiplication of ornamentals like orchids genera, carnation, roses, chrysanthemum and plantation crop like banana and cardamom..The compounds responsible for the memory enhancing effects of Bacopa monniera are triterpenoid saponins called bacosides .In the Ayurvedic Materia Medica, Bacopa has been recognized for its brain enhancement characteristics. Bacopa monnieri, a member of the Scrophulariaceae family, is a small, creeping herb with numerous branches, small oblong leaves and light purple flowers. In India and the tropics it grows naturally in wet soil, shallow water and marches. Brahmi (Bacopa monnieri) (Linn) familyScrophulariaceaeis also known as Madhyarasayana in Ayurveda as it increase mental-clarityand brain stimulating action It also possesses anti-inflammatory, analgesic, antipyretic, epilepsy, insanity, anticancer and antioxidant activities, saponins such as bacosides A, B, C and D which are the active triterpenoid principles and known as "memory chemicals".Only  a  very limited research has been  carried  out  on the plant,  under  the present study  assumes singular  significance and it is supposed to contribute a great deal to the existing literature. The present paper highlights the morphogenic response of various auxins and cytokinins on Bacopa monniera.

Role of Essential Medicines in framing National Medicine Policy – An Approach from Concept to Clinic

About Authors: Ashish Chauhan.
Department of Pharmaceutical Sciences,
Lovely Professional University,
Jalandhar, Punjab.

The essence of essential medicines underlies in satisfying the priority health care needs of the population. The selection of essential medicines is one of the core principles of a national medicine policy as it helps to set primacies for all the aspects of the pharmaceutical system and helps in the maintenance of a proficient healthcare system globally. Devising of essential medicines and national medicine policy is fast emerging as global concept of high relevance to the public irrespective of their nationalities. The prevalence of dreaded diseases like HIV, cancer, and a worldwide upsurge in case of malaria, tuberculosis and other infectious diseases with an addendum problem of multidrug resistance has further augmented the relevance of essential medicines and formulation of a rigid comprehensive national drug policy under the endorsement of WHO medicine strategy andimplementing the same under a complete implementation plan or master plan. This review emphasis the development and selection of essential medicines along with Indian scenario. This review also emphasis the mission, objectives, components, devising and implementation of essential medicine policy along with WHO tenets and regulations.