March 2016 ARTICLE LIST >>
PharmaTutor (March- 2016)
Print-ISSN: 2394 - 6679
e-ISSN: 2347 - 7881
(Volume 4, Issue 3)
Received On: 02/10/2015; Accepted On: 15/10/2015; Published On: 01/03/2016
AUTHORS:
Saka OS*, Olayode AA, Adelodun ST
Department of Anatomy and Cell Biology,
Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
* olusolasaka1@gmail.com
ABSTRACT: This study was designed to evaluate the effects of garlic extracts on the morphology of myocardium of left ventricle of the heart and also determine the body weight and relative weight of the organs on high salt fed adult Wistar rats. Twenty-five healthy female Wistar rats weighing 130-180 g were randomly assigned into 5 groups of 5 rats each (Groups A, B, C, D and E). Rats in group A was fed with standard laboratory pellets, while groups B, C, D and E were fed on the high-salt diet for five weeks. Thereafter, daily administration of 50 mg kg-1, 100 mg kg-1 and 150 mg kg-1 of the garlic extract were giving orally to groups C, D and E respectively for 3 weeks. The rats were sacrificed under ketamine anesthesia (5mg/kg i.m). The left ventricle of the heart was excised, processed routinely in paraffin wax and stained with routine special stained. Result showed significant decreased (p<0.05) in weight of all high salt fed groups when (F=46.90, p<0.05) compared with control. Whereas, treatment with garlic extract help in weight management in high salt fed+garlic extract treated groups and also no significant difference (p<0.05) in the relative heart weight when (F=1.773, p<0.05) compared the control with other groups. Histological results revealed morphological alterations in the left ventricle in high salt fed group. In conclusion, Garlic extract has ameliorative property at the level of 100 mg kg-1 or 150 mg kg-1 of the extract on high salt fed induced cardiac injury.
How to cite this article: Saka OS, Olayode AA, Adelodun ST; Effects of Garlic extracts on the myocardium of left ventricle of the heart of high salt fed adult wistar rats; PharmaTutor; 2016; 4(3); 22-27
[ABSTRACT WITH CITATION] [VIEW AS HTML]
REFERENCES:
1. Mukherjee S., Lekli I., Goswami S. and Das D. K; Freshly crushed garlic is a superior cardioprotective agent than processed garlic; J. Agric. Food Chem; 2009; 57(15); 7137-7144.
2. Harauma, A. and T. Moriguchi; Aged garlic extract improved blood pressure in spontaneously hypertensive rats more safely than raw garlic; J. Nutr; 2006; 136(3 Supp); 769S-773S.
3. Dhawan V. and Jain S.; Garlic supplementation prevents oxidative DNA damage in essential hypertension; Mol. Cell Biochem; 2005; 275(1-2); 85-94.
4. Reuter H.D.; Allium Sativum and Allium ursinum. Part 2: pharmacology and medicinal application; Phytomedicine; 1995; 2(1); 73-91.
5. Banerjee S.K. and Maulik S.K.; Effect of garlic on cardiovascular disorders: a review; Nutr J; 2002; 1; 4-30.
6. Pedersen K. E., Neilsen J., Klitgaard R. and Johansen N.A.; Sodium content and sodium efflux of mononuclear leucocytes from young subjects at increased risk of developing countries; American Journal of Hypertension; 1990; 3(3); 182-187.
7. Barthan N., Laurant P., Hayoz D., Fellmann D., Brunner H.R. and Berthelot A.; Magnesium supplementation and DOCA acetate- salt hypertension: Effect on arterial mechanical properties and on activity of endothelin-1; Canadian Journal of Physiology and Pharmacology; 2002; 80(6); 553-561.
8. Bragulat E. and de la Sierra A.; Salt intake endothelial dysfunction and salt-sensitive hypertension; J. Clin. Hypertens; 2002; 4(1); 41-46.
9. Obiefuna P.C.M. and Obiefuna I. P.; Salt induced hypertension in rats alters the response of isolated aortic rings to cromakalim; West Indian Med. J; 2001; 50(1); 17-21.
10. Feng J.H., Michel B. and Graham A.M.; Nutrition in cardiovascular disease: salt in hypertension and heart failure; European Heart Journal; 2011; 32(24); 3073-3080.
11. Resende M.M. and Mill J.G.; Effect of high salt intake on local reninangiotensin system and ventricular dysfunction following myocardial infarction in rats; Clin Exp Pharmacol Physiol; 2007; 34(4); 274-9.
12. Ahn J., Jasmina V., Michel S., Dinko S. and Edward D.F.; Cardiac structural and functional responses to salt loading in SHR; Am J Physiol Heart Circ Physiol; 2004; 287(2); H767-H772.
13. Yu H.C., Burrell L.M., Black M.J., Wu L.L., Dilley R.J., Cooper M.E. and Johnston C.I.; Salt induces myocardial and renal fibrosis in normotensive and hypertensive rats; Circulation; 1998; 98(23); 2621-2628.
14. Banerjee S.K., Mukherjee P.K. and Maulik S.K.; Garlic as an antioxidant: the good, the bad and the ugly; Phytother Res; 2003; 17(2); 97-106.
15. Sharifi A.M., Darabi R. and Akbarbo N.; Investigation of antihypertensive mechanism of garlic in 2KIC hypertensive rat; Journal of Ethnopharmacology; 2003; 86(2-3); 219-224.
16. Palmer B.F.; Managing hyperkalemia caused by inhibitors of the rennin-angiotensin aldosterone system; N. Engl. J Med.; 2004; 351(6); 585-592.
17. Cordaillat M., Rugale C., Casellas D., Mimran A. and Jover B.; Cardiorenal abnormalities associated with high sodium intake: correction by spironolactone in rats; Am J Physiol Regul Integr Comp Physiol; 2005; 289(4); R1137-1143.
18. Ziegelho F.F., Mihalovicova B., Arnold N., Marx G., Tannapfel A., Zimmer H.G., et al; Effects of salt loading in various therapies on cardiac hypertrophy and fibrosis in young spontaneously hypertensive rats; Life Sci; 2006; 79(9); 838-46.
19. Varagic J., Frohlich E.D., Susic D., Ahn J., Matavelli L., Lo´pez B., et al; AT1 receptor antagonism attenuates target organ effects of salt excess in SHRs without affecting pressure; Am J Physiol Heart Circ Physiol; 2008; 294(2); H853-858.
20. Ofem O., Ani E., Okoi O., Effiang A., Eno A. and Ibu J.; Effect of Viscum album (mistletoe) extract on some serum electrolytes, organ weight and cytoarchitecture of the heart, kidney and blood vessels in high salt fed rats; The Internet Journal of Nutrition and Wellness; 2006; 4 (1).
21. Walter S., Wiggins M.D., Glayton H., Manry M.D. and Richard H.; The effect of salt loading and salt depletion on renal function and electrolyte excretion in man; Neuropeptide; 2001; 35(3-4); 181-188.
22. Coelho M.S., Passdora M.D., Gasparretti A.L., Bibancos T., Prada P.O., Furukawa L.L., Furukawa L.N., Fukui R.T., Casarini O.E., Saad M.J., Luz J., Chiavegatto S., Dolnikoff M.S. and Heimann J.C.; High- or low-salt diet from weaning to adulthood, effect on body weight, food intake and energy balance in rats; Nutr. Metab. Cardiovasc. Dis.; 2006; 16(2); 148-155.
23. Ohaeri O.C.; Effect of garlic oil on the levels of various enzymes in the serum and tissue of streptozotocin diabtic rats; Biosci Rep.; 2001; 21(1); 19-24.
24. Ferreira F.C., Abreu L.C., Valenti V.E., Ferreira M., Meneghini A., Silveira J.A., et al; Anti-hypertensive drugs have different effects on ventricular hypertrophy regression; Clinics; 2010; 65(7); 723-728.
25. Kilikdar D., Mukherjee D., Dutta M., Ghosh A.K., Rudra S., Chandra A.M. and Bandyopadhyay D.; Protective effect of aqueous garlic extract against lead-induced cardiac injury in rats; Journal of Cell and Tissue Research; 2013; 13(3); 3817-3828.
26. Ichihara S., Noda A., Nagata K., Obata K., Xu J., Ichihara G., Olkawa S., Kawanishi, Yamada Y. and Yokota M.; Pravastatin increases survival and suppresses an increase in myocardial matrix metallo proteinase activity in rat model of heart failure; Cardiovascular Research; 69(3); 2006; 726-735.
27. Cingolani O.H., Yang X.P., Cavasin M.A. and Carretero O.A.; Increased systolic performance with diastolic dysfunction in adult spontaneously hypertension rats; Hypertension; 2003; 41(2); 249-254
28. Kescienly J., Klussendorf D. and Latza R.; The antiatherosclerotic effect of Alliums sativa; Atherosclerosis; 1999; 144(1); 237-49.
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