Articles

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR QUANTIFICATION OF MEBENDAZOLE IN API AND PHARMACEUTICAL FORMULATIONS

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ABOUT AUTHORS
Durgesh Rameshlal Parakh1*, Jayshri K. Madagul1, Harshad Rajendra Mene2, Moreshwar P. Patil1, Sanjay J. Kshirsagar1
1
Department of Pharmaceutics,
MET’s Institute of Pharmacy, Adgaon, Nashik, Maharashtra, India.
2 Department of Pharmaceutics,
Government College of Pharmacy, Osmanpura, Aurangabad, Maharashtra, India.
*aryanparakh57@gmail.com

ABSTRACT
A new simple and sensitive RP-HPLC method was developed and validated for quantification of Mebendazole in Active Pharmaceutical Ingredient (API) and pharmaceutical formulation.The gradient RP-HPLC method was developed on Agilent (India) C18 250 × 4.6 mm, 5 μ column using mobile phase as acetonitrile: water pH 3.0 with orthophosphoric acid (90:10 v/v) at a flow rate of 1 mL/min and detection was carried out at 234 nm using UV-Visible detector (UV 3000 M). The method was validated linearity, limit of detection, limit of quantification, precision, ruggedness, robustness, accuracy and specificity were found to be satisfactory. The method was found to be linear in the concentration range of 20-100 μ/mL with correlation coefficient of 0.999. The method was validated according to the ICH guidelines and was proved to be specific, linear, accurate, precise and economical for the analysis of Mebendazole.


A SHORT REVIEW ON RHEUMATOID ARTHRITIS (RA)

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ABOUT AUTHORS
Sabya Sampad Swain, Nadugopal Bhuyan, Chaitanya Prasad Meher
Gayatri institute of science & technology (GIST), Gunupur, Rayagada, Orissa
swainsabhya@gmail.com

ABSTRACT
Rheumatoid arthritis is most common disease in India as well as outside India. The Centers for Disease Control and prevention estimates that more than 40 million Americans have some form of arthritis or chronic joint disorder out of them 7 million Americans suffer from arthritis in its most debilitating forms. Arthritis foundation classified rheumatic diseases as inflammatory disorders affecting more & more individuals than any chronic illness. Osteoarthritis is the most common form of arthritis in the United States, affecting about 12% of Americans between the ages of 25 and 74. Rheumatoid arthritis is thought to affect well over 2 million Americans (two to three times more females more than males), whereas juvenile arthritis affects 71,000 children under 16 years of age, 61,000 of whom are females. The presented article is concern with a brief idea about rheumatoid arthritis.


DATA INTEGRITY AND WORLDWIDE REGULATORY GUIDANCE

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ABOUT AUTHORS
Rohit A. Patil1*, Shruti N.Patil2

Department of Regulatory Affairs,
Supreme Pharma Healthcare Pvt. Ltd. Mumbai, MH, India
* rohitpharma3250@gmail.com

ABSTRACT
Good storage of data & record management are critical elements of pharmaceutical quality system. Data integrity refers to maintaining & assuring the accuracy & consistency of data over its entire life –cycle in compliance with its applicable regulatory requirements.
Data integrity is mandatory for the regulated pharmaceutical industry, as processing and disposition decisions regarding product quality, safety, efficacy, purity, and compliance with the applicable regulatory requirements are made based on data that is recorded and reported. Data integrity risk should be assessed, mitigated, communicated & reviewed throughout the data life cycle. Healthcare industries should be designed Record-keeping methodologies and systems, in a way that encourages compliance and assures data quality and reliability.


REVIEW STUDY ON FACTORS AFFECTING THE PRESCRIPTION PATTERN OF PHYSICIANS

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About Authors
Amoah-Binfoh Kenneth1*, Pradhyuman Singh Lakhawat1, Archana Chandra2
1 SHIATS, Allahabad, UP, India
2 Dept. of Human Resources, SHIATS, Allahabad, UP, India
* geokin08@gmail.com

ABSTRACT
Prognosis the medical outcome of a corrective prediction, physicians become aware of a particular drug through promotional strategies. If all relevant information received is positive, the drug may be tried on few patients’. As a matter of fact if the outcomes of the trials are acceptable physicians will shift and may be more interested to prescribe the drugs consistent. Prescribing drugs is a standard component of most physicians’ practices. It is an important area of practice that requires appropriate knowledge, skill and professional judgment. To improve patient safety when prescribing, these qualities set out expectations for physicians who prescribe drugs. However in connection with the physician’s prescription pattern, Indian pharmaceutical industry is at a turning point. The pharmaceutical firms have to change their strategies for the challenges ahead. Quality systems, innovation, manufacturing and marketing competence are associate factors that affects physicians prescription pattern. The efficacy of drug discovery process has been reported to have suffered a major transformation. New drug discovery has to be strictly adhere to the tight intellectual property rights to ensure effective and efficient, prescriptions and protections. Further clinical and behavioral characteristics of the patient, research and the pressures from drug firms, high curing effect; habitual or non-habitual choice, behavior pattern of patients, peer influence of physicians (community of physicians) and education and advertising are reviewed factors affecting physician’s prescription pattern.


DTK OF MEDICINES FROM BIO-PIRACY: ITS CONSCIENTIOUSNESS BY TKDL OF INDIA

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ABOUT AUTHORS:
NG Dhawan*1, Manisha Mavai2, Priyanka Bishnoi3, Raaz K Maheshwari3
1Amity Institute of Environmental Sciences, Amity University,  Noida, UP, India
2Department of Botany Physiology, AIIMS, Jodhpur, Rajasthan, India
3Department of Botany & Biotechnology, University of Rajasthan, Rajasthan, India
4Department of Chemistry, SBRM Govt PG College, Nagaur, India
*rkmgreenchem.jaipur@gmail.com

ABSTRACT
The genetic resources and indigenous communities traditional knowledge of developing countries is being exploited by the Developed countries in the name of patents for inventions that are derived from genetic  resources. The exploitation of genetic resources and indigenous communities traditional knowledge by the developed countries in the name of patents on the inventions derived from those genetic resources is actually bio-piracy.  Protection of the TK of the local and indigenous communities appears to be one of the most debatable and complex issues. This paper brings out the role of domestic IPR constitution that looks to protect traditional system of medicine and associated TK in Indian sub-continent. Further, it attempts to validate the necessity of introduction of a national TKDL, which can be utilized as an evidence of the 'prior art' by the examiners of patent offices, nationally as well as internationally. Thus, it aims to address the concerns relating to the creation of TKDL in India.


PHARMACOLOGY OF COMBINED RANOLAZINE AND AMIODARONE HYDROHLORIDE THERAPY TO TREAT ATRIAL FIBRILLATION

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ABOUT AUTHORS:
Patel  Vishakha D*, Raj Hasumati A, Gheewala Nirav K
Department of Quality Assurance,
Shree Dhanvantary Pharmacy College, Kim, Surat
Department of Quality Assurance,
vishuk7293@gmail.com, drharaj@yahoo.com

ABSTRACT:
This review article presents the pharmacology of combined Amiodarone Hydrochloride and Ranolazine therapy specially in Atrial Fibrillation. Amiodarone Hydrochloride is Anti Arrhythmic agent. Ranolazine is a Anti Anginal agent. The Antianginal agent was used in Ischaemia. Amiodarone Hydrochloride is Antiarrhythmic agent and use in Arrhythmia and Atrial fibrillation. If Amiodarone Hydrochloride is administered in large quantities, the condition of patient becomes worse by occurrence of adverse effect of Thyroid toxicity due to presence of Iodine moiety in Amiodarone. The use of Ranolazine in combination with Amiodarone Hydrochloride has been proved to provide beneficial effect in Atrial Fibrillation. The combination therapy has fewer adverse effects. The mechanism of Amiodarone Hydrochloride and Ranolazine is quite different. Amiodarone Hydrochloride inhibit potassium channel and inactivated-state blocker of cardiac sodium channel while Ranolazine inhibit late inward sodium current (INa) in cardiac cell and activated-state blocker of cardiac sodium channel. The combination of both have decrease dose dependent side effect of Amiodarone Hydrochloride. Both the drugs were approved by US government and has been used in Atrial Fibrillation in US.The main objective of this review article is to provide pharmacological information of combined therapy of Amiodarone Hydrochloride and Ranolazine to researcher in development of combined dosage form of this.


A REVIEW ON: PHYSICOCHEMICAL EVALUATION OF AYURVEDIC MINERAL DRUG TANKAN BHASMA

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ABOUT AUTHORS:
*Shaikh S.M., Doijad R.C., Shete A.S., Sankpal P.S.
Shree Santkrupa College of Pharmacy,
Ghogaon, Karad Maharashtra 415111 (India)
*sabirmshaikh17@gmail.com

ABSTRACT:
Tankan bhasma which has been used since very long time in Ayurveda. Tankan bhasma purification is done by the mand agni. Evaluation of physic-chemical property of Tankan bhasma. We are studing toxicological study of Tankan (Borax). Toxicological study on eyes, skin, inhalation, ingesion, carcinogenicity, reproductive or developmental, target organs, also signs and symptoms of exposure. We are studied on the first aid treatment if hazards due to contacts with eyes, Ingestion, and skin. Various uses of Tankan (Borax) bhasma we are studying. Also we are studying the analytical evaluations. Such as Tankan (Borax) bhasma evaluation containing moisture content, organic contain, Nishchandratva, Nishchandratva, Varitaratvam, Rekhapurnatvam, Unama test, Nirchandrata, Amla pariksha, Avami, Anjana Sannibha, X-ray diffraction i.e XRD study, also the Scanning Electron Microscope that is SEM, this all methods we are using for the evaluation of Tankan (Borax) bhasm.


ZERO ORDER AND FIRST ORDER DERIVATIVE SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND KETOROLAC TROMETHAMINE IN SIMULATED TEAR FLUID

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ABOUT AUTHORS:
Shashank Nayak N*1, U Srinivasa2, Shwetha S Kamath K3, Shabaraya AR4
1Dept of Pharmaceutics, Pacific university, Udaipur, Rajasthan
2Dept.of Pharmacognosy, Srinivas college of pharmacy, Mangalore, Karnataka
3Dept. of Pharmaceutics, Srinivas college of pharmacy, Mangalore, Karnataka
4Principal, Srinivas College of Pharmacy, Mangalore, Karnataka
shashanknayak87@gmail.com

ABSTRACT
In this current work simultaneous estimation of Moxifloxacin HCL and Ketorolac tromethamine in simulated tear fluid was performed by using UV spectroscopy. The zero order derivative spectroscopy revealed that Moxifloxacin HCL had a λ max of 288 nm in Simulated tear fluid (STF) and Ketotorolac tromethamine had λ max of 322nm respectively. The linearity was found in the range of 2-10 µg/ml for Moxifloxacin HCL and 2-14µg/ml for ketorolac tromethamine.  The R2 value was found to be 0.998 and slope y=0.089 for Moxifloxacin HCL and R2 =0.998 and slope y=0.051 for Ketorolac tromethamine. The zero order spectrum was deravatized to first order derivative spectra for both the above mentioned drug. The first order derivative spectra of Moxifloxacin HCL and ketorolac tromethamine indicated that it had a zero crossing point (ZCP) at 286 nm and 328 nm. The absorbance of the mixture containing MOXI and KETO was found out at 286 nm and 328 nm which showed linearity and obeyed beers lamberts law.


ACTIVITIES OF AQUEOUS EXTRACTS OF MANGIFERA INDICA ON PARASITAEMIA LEVEL AND BLOOD PROFILE OF PLASMODIUM BERGHEI - INFECTED ALBINO MICE

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ABOUT AUTHORS
*Olayode, Ahmed A1; Saka, Olusola S1; Ajayi, Olusoji C2; Agbaje, Mojeed A1
1Department of Anatomy and Cell Biology, Faculty of Basic Medical Science
2Department of Pharmacognosy, Faculty of Pharmacy
Obafemi Awolowo University, Ile-Ife, Nigeria
olayodeahmed01@gmail.com

ABSTRACT
This study was carried out to assess the effect of Mangifera indica on the percentage parasitaemia and some blood parameters of Plasmodium – infected Albino mice. Forty-two male albino mice, weighing between 20 and 25 g, were used for this research. The mice were randomly assigned into six groupsA, B, C, D, E and F of seven mice each. Group A was the negative control, group B was positive control. Mice in groups C, D and E were orally administered with Mangifera indica extract (50 mg/kg, 100 mg/kg and 150 mg/kg body weight) respectively for 5 days starting from the 4th day of inoculation while mice in group F were orally administered with Artesunate for 5 days (3 mg/kg b.w on the first day and 1.5 mg/kg b.w for the next 4 days) starting from the 4th day of inoculation. Blood smears were taken every 2 days to check the parasitaemia level for 14-day post-treatment withdrawal period. The extract has a dose dependent reducing effect on the level of parasitaemia. It also showed that the increase in the rate of reticulocytosis and consequent increase in the number of red blood cells in a dose dependent manner.


BIO-MARKERS AND BIO-SENSORS: UNRAVELLING THE MAJOR MECHANISTIC INTERVENTIONS IN CANCER PROGNOSIS

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ABOUT AUTHOR
Rajesh Tiwari
Junior Technical Assistant
Pharmaceutical Science and Natural Products
Central University of Punjab, Bathinda
rajesh.tiwari851@gmail.com

ABSTRACT
Biosensors are a tool that combines the biochemical element with a transducer that generates an indication for the identification of disease. Numbers of biosensors are used in different medical application. Biomarker and Biosensors play an important role for early stage exposure of cancer. It is a cost-effective, increased assay speed, resiliency, the ability for multi-target analysis method. Genomic and proteomic molecular tools are also used for the exposure of tumors. These methods produce a molecular mark that provides a new opening for utilizing biosensors and biomarkers. As with many disease conditions, it is not easy to find exact and responsive markers that are associated with only one marker. Different numbers of molecular signatures are used for the diagnosis of cancer, such as proteins, peptides, overexpression gene and gene mutation. This review provides a general idea of the biosensors and biomarkers technology that is currently developed and researched for cancer markers and diagnosis.


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