You are herePharmacology Articles

Pharmacology Articles


IN-VITRO SCREENING OF DHATURA INOXIA FOR ITS ANTI-MALARIAL ACTIVITY AGAINST P. FALCIPARUM

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
S.O. Pratap1,2, Ruchi Gaur3, R.Stephan4, J.L bhatt3, C.R. Pillai1, Usha Devi1
1Department of  Parasitology, Malaria Research Center, New Delhi
2Department of  Biotechnology, SR Group of Institutions, Jhansi, UP
3School of Life Science, ITM University Gwalior, MP
4Department of Botany, Government P.G. College Ariyalur, Tamilnadu, India
dixitshivom@gmail.com

ABSTRACT
The natural products are important source of biologically active compounds having potential for developing a novel malaria drug. Malaria is one of the most important infectious vector born parasitic disease caused by Coccidian protozoan parasite of genus Plasmodium carried out by mosquito, invade red blood cells. Malaria is thousand times more complex than any disease. In the current study, a natural ingredient of herb; Dhatura Inoxia is analyzed, for its anti-malarial activity by preparing crude extract of water and ethanol, analyzed on schizont stage of plasmodial life cycle against two Chloroquine strains: sensitive and resistant of Plasmodium falciparum isolates. The results revealed that IC50 values of crude water extract was recorded as; 71± 1.41µg/ml and 105±7.07 while as the ethanol crude extract produced IC50 concentrates: 52±3.53 µg/ml and 59.5± 70 µg/ml against sensitive and resistant P. f. isolates, respectively. The results showed that this herb plant possess significant antiplasmodial activity in terms of IC50 as recorded in this study.


ASPERGILLOMA SYNDROME

ABOUT AUTHOR:
Akshay Rajgaria, Prajna Tripathy
Kanak Manjari Institute of pharmaceutical Sciences.
Rourkela, Orissa
akshaykrish2007@gmail.com

ABSTRACT:
An aspergilloma, also known as a mycetoma or fungus ball, is a clump of fungus which exists in a body cavity such as the lung. It is associated with the Aspergillus species, but Zygomycota and Fusarium may also form similar structures


INTERLEUKINS IN THERAPEUTICS

{ DOWNLOAD AS PDF }

ABOUT AUTHOR:
Anjan Khadka
Department of Pharmacology,
AFMC, Pune, India
anjankhadka14@yahoo.com

ABSTRACT:
Interleukins are a subset of a larger group of cellular messenger molecules called cytokines, which are modulators of cellular behaviour. On the basis of their respective cytokine profiles, responses to chemokines, and interactions with other cells, these T-cell subsets can promote different types of inflammatory responses. During the development of allergic disease, effector TH2 cells produce IL-4, IL-5, IL-9, and IL-32.  IL-25, IL- 31, and IL-33 contributes to TH2 responses and inflammation. These cytokines have roles in production of allergen-specific IgE, eosinophilia, and mucus. ILs have role in therapeutics as well as diagnosis and prognosis as biomarker in various conditions. Therapeutic targeting of the IL considered to be rational treatment strategy and promising biologic therapy.


A REVIEW ON INTERSTITIAL CYSTITIS SYNDROME (ICS)

{ DOWNLOAD AS PDF }

About Authors:
M Sushma*, TVV Vidyadhar, R Mohanraj, M Babu
Department of Pharmacy Practice, Raghavendra Institute of Pharmaceutical Education & Research (RIPER),
K R Palli Cross, Near S.K University, Anantapuramu District, Andhra Pradesh, INDIA.
*sushma.banthi@gmail.com

Abstract:
Interstitial cystitis (IC) is an irritative bladder syndrome of multifactorial aetiology and is said to be one of the several conditions that causes bladder pain, which is most commonly seen in women than in men. It is of unknown aetiology. But some factors like autoimmune disorder, Genetic predisposition, Gynaecologic surgery in the past, History of recurrent UTIs. The body's immune system attacks the bladder, similar to other autoimmune conditions. The urologist may use a special scope called a cytoscope to look inside your bladder for inflammation, pinpoint bleeding. Management of IC is done by oral therapy of pentosanpolysulphate, Tricyclic anti-depressants, Anti-histamines, Non-steroidal anti-inflammatory drugs, Bladder analgesics. Whereas intravesical therapy Dimethyl sulphoxide, heparin, Bacillus calmette-Guerin


KNOCKDOWN (MICRO-RNA INHIBITING TECHNIQUE) TECHNIQUE FOR EVALUATION OF ALPHA-1A ADRENERGIC RECEPTOR AND P2X1 RECEPTOR FUNCTIONS

{ DOWNLOAD AS PDF }

About Authors:
Emanual Michael Patelia*, Emma Spiking
University of Bedfordshire, England,
United Kingdom, LU1 3JU
ricky.emanual@gmail.com

Abstract
The new approach regarding the knocking down the protein expression by the use of shmRNA complimentary to the mRNA sequence of the respected protein expression have a major impact on the research area. The knockdown of P2X1 and ADRA1A genes have no effect on chromosomal DNA but have a lack of effect on expression of P2X1 and α1A receptors. The produced genetically engineered male mice can be of 100% infertile. The simple PCR (polymerase chain reaction) based approach can be used for the generation of knockdown cell lines. The Animals should have the treatments according to the guidelines mentioned under international society for applied ethnology. The SIRION biotech have shown the managed shRNA knockdown of about 90% significance interval.


A REVIEW: ATHEROSCLEROSIS & ITS TREATMENT

{ DOWNLOAD AS PDF }

About Author:
Mr. Yogesh K. Patil
Lecturer, Department of Pharmacology,
Shree Mahavir Institute of Pharmacy,
Nashik.
yogesh_kpatil85@yahoo.co.in

Abstract:
Atherosclerosis is deposition of fatty substances into the inner wall of blood vessels. Due to deposition of lipid material into blood vessels there is restricted flow to blood to various organs like kidney, heart, lungs etc. To overcome this effect various drugs available in market like statins, fibrates etc. There is also way to clean lipid from inner lining of blood vessels through surgery which is last step of treatment. There are mainly two surgeries available that is bypass surgery of heart and angioplasty.


ANTI-DIABETIC ACTIVITY OF GOSSYPIUM HERBACEUM BY ALLOXAN INDUCED MODEL IN RATS

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
C Velmurugan*1, Anurag Bhargava2
1Department of Pharmacology, Research scholar, Institute of Pharmaceutical sciences and Research center, Bhagwant University, Sikar road, Ajmer, Rajasthan, India-305004.
2Department of Pharmacognosy, CH. Devi lal College of Pharmacy, Bhagwangarh, jagadhri, Haryana-135003.
velu0906@gmail.com

ABSTRACT
The hypoglycemic and hypolipidemic effect of ethyl ether and ethanol extracts of Gossypium Herbaceum (200mg/kg) leaves was evaluated with measurements including, blood glucose level and biochemical parameters. The extracts of the leaves was tested for its efficacy in alloxan-induced diabetic rats. Animals were induced for diabetes with Alloxan (150 mg/kg of body weight- i.p.). The extracts of Gossypium Herbaceum administered daily. The extracts were also evaluated for acute oral toxicity studies and its effect on different biochemical parameters. The extracts showed significant (p<0.01) antihyperglycemic and hypolipidemic activity as compared to diabetic control. The extracts show beneficial effects on blood glucose level in alloxan model. It also reduces the elevated biochemical parameters such as triglycerides (TGL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), Total Cholesterol (TC) and increased the reduced level of high density lipoprotein (HDL), which might be due to presence of flavanoids, tannins, alkaloids and phenolic compound present in that extract. Thus extracts of Gossypium Herbaceum could serve as good oral hypoglycemic agents and seems to be promising for the development of phytomedicines for diabetes mellitus associated cardiac disorders.


SEX DETERMINATION USING THE ADVANCED OMIC TECHNIQUES: POLYMERASE CHAIN REACTION AND SDS PAGE GEL ELECTROPHORESIS

{ DOWNLOAD AS PDF }

About Authors:
Emanual Michael Patelia*, Emma Spiking
Department of Pharmacology,
University of Bedfordshire, England,
United Kingdom, LU1 3JU
ricky.emanual@gmail.com

Abstract:
The polymerase chain reaction is widely used technique for the amplification of target nucleotides for the gene of interest. Amplification of the SRY gene on the Y chromosome by PCR and the identification by electrophoresis has a major role in sex determination. In the proposed method, the house keeping primer glyceraldehydes phosphate dehydrogenase (GAPDH) as a marker of both total and DNA and mitochondrial RNA and SRY gene should be used for the identification of the unknown samples. In the SDS PAGE gel electrophoresis, two bands are noticeable; first band at 218 bp for GAPDH and second band at 741 bp for SRY gene. The sex chromosomes has a important role in the etiology of normal sexual development. Biopotential gonal needs two chromosomes to the development of ovary. The existence of SRY gene located on the Y chromosome, a testis is developed. The sex ambiguity can be developed by the discodance between chromosomal sex and appearance of the external genitalies.


PROBIOTICS - A REVIEW

{ DOWNLOAD AS PDF }

About Authors:
V.Sankar1*, Akash kurian2
*1Department of Pharmacy Practice, PSG College of Pharmacy, Coimbatore.
2PHARM.D Intern, PSG College of Pharmcy, Coimbatore.
*akash.kurian8@gmail.com

Abstract:
The objective of review is to outline the probiotics use in medicine and to give insight in to the field for different applications. The use of probiotics in treatment regimen is increasing. Probiotics are live organisms and prebiotics are components of food that are not otherwise easily digested by humans and these food components essentially feed beneficial bacteria in the gut. Probiotics can be formulated to many different types of products including drugs, foods and dietary supplements. Species of lactobacillus and bifidobacterium are most commonly used as probiotics. Probiotics are intended to assist the body’s naturally occurring gut mitochondria. Some probiotic preparations have been used to prevent diarrhea caused by antibiotics or as part of treatment for antibiotic related dysbiosis. This article focus on the probiotics use,  clinical trials and its future.


A REVIEW: EFFECT OF DIABETES ON VASCULAR ENDOTHELIUM

{ DOWNLOAD AS PDF }

About Authors:
Yogesh K. Patil
Lecturer, Department of Pharmacology,
Shree Mahavir Institute of Pharmacy,
Nashik.
yogesh_kpatil85@yahoo.co.in

Abstract:
Endothelial dysfunction is mainly observed in diabetics. The main role of endothelium is to control the tone of the vascular smooth muscle through the production of vasodilator mediators like nitric oxide (NO), prostacyclin, and endothelium-derived hyperpolarizing factor (EDHF). Impaired endothelium-dependent vasodilation has been observed in vascular beds of different animal models of diabetes and in humans with type 1 and 2 diabetes. Hyperglycaemia causes repeated acute changes in intracellular metabolism and causes activation of polyol pathway, activation of protein kinase C, advanced glycation end product and increased oxidative stress.


FIND MORE ARTICLES

Subscribe to RSS headline updates from:
Powered by FeedBurner