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  • QUANTITATIVE DETERMINATION OF ASENAPINE MALEATE USING REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

    About Authors:
    T.R.PARTHASARATHI*, M.VANITHA SRI.
    BIOANALYTICAL DEPARTMENT,
    QUEST LIFE SCIENCES PVT. LTD, SDF III, MEPZ,
    TAMBARAM, CHENNAI- 600 045, INDIA.
    *parthu_14@yahoo.co.in

    ABSTRACT :
    A novel isocratic reverse-phase high performance liquid-chromatography method for determination of asenapine maleate was developed and validated after optimization of various chromatographic conditions. Samples were separated on a waters x-terra C18 (100 mm × 4.6 mm, 3.5 μ) analytical column. The mobile phase used was acetonitrile: 0.1M phosphate buffer (pH 3.2) 65:35%v/v operated at 30 °C column oven temperature was pumped at a flow rate of 1.0 mL min−1 and the column eluents were monitored at a wavelength of 272 nm. When sample was injected into the Finnigan surveyor high performance liquid-chromatography system through Finnigan surveyor auto-sampler injector, separation was achieved within 5.0 min. The present method demonstrated was validated with the acceptable values for selectivity, linearity (within the expected concentration range (10–50 μg mL−1; r2 > 0.999)), recovery (>95%), precision (%RSD < 2.0), sensitivity (limit of detection: 1.85 µg mL−1 and lower limit of quantification: 2.34 µg mL−1), robustness, and ruggedness.

  • Anti-inflammatory activity oflic extract of R Methanooot of Cissampelos pareira on Carragenin induced rat paw edema

    About authors:
    Gourab Saha*1, Pankaja Senapati1, Narahari Sahu2, Dr. Sambit Parida3
    1. Department of Pharmaceutics, College of Pharmaceutical Sciences, Mohuda, Berhampur – 2, Orissa, India.
    2. Department of Pharmacology, College of Pharmaceutical Sciences, Mohuda, Berhampur – 2, Orissa, India.
    3. Department of Pharma analysis, College of Pharmaceutical Sciences, Mohuda, Berhampur–2, Orissa, India.

    *gourab.pharma2012@gmail.com

    Abstract
    This study investigated the anti-inflammatory activity of the methanolic extract of Cissampelos pareira (Abuta) in male albino rats after intramuscular administration. This was done using the carragenin-induced paw edema method. Methanolic extract of Cissampelos pareira showed significant anti-inflammatory activity similar to ibuprofen and indomethacin.

  • SIMULTANEOUS DETERMINATION AND VALIDATION OF TRIAMTERENE AND BENZTHIAZIDE BY DUAL WAVELENGTH AND RATIO DERIVATIVE METHOD IN BULK AND PHARMACEUTICAL FORMULATION

    About Authors:
    Megana.H.S*, A.Satish Kumar Shetty, Anil Kumar S.M
    *Department of Pharmaceutical Analysis,
    National College of Pharmacy, Balraj Urs road,
    Shimoga-577201,
    Karnataka, India.
    megana.leo@gmail.com

    ABSTRACT
    A novel, accurate, precise, sensitive, economic and rapid spectrophotometric methods has been developed and validated for simultaneous estimation of Triamterene and Benzthiazide in bulk and pharmaceutical dosage form. Triamterene and Benzthiazide showed absorption maxima at 363 nm and 283 nm in ethanol, which is used as solvent. In the present study, Area Under Curve method (Method A) developed, employedthe measurement of area at selected analytical wavelength ranges. Two analytical wavelength ranges selected were 358nm to 368nm nm (lmax of Triamterene is 363nm) and 278nm to 288nm (lmax of Benzthiazide is 283nm) for the estimation of Triamterene and Benzthiazide respectively.  Ratio derivative method (Method B) employed the measurement of amplitudes of Triamterene and Benzthiazide at their respective selected wavelengths. Two wavelengths selected were 359.2 nm and 300.4 nmfor the estimation of Triamterene and Benzthiazide respectivelybased upon divisor method.  Linearity was observed in the concentration range of 6-30 μg/ml and 3-15 μg/ml for Triamterene and Benzthiazide respectively. The recovery studies ascertained the accuracy of the proposed method and the results were validated as per ICH guidelines.

  • REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY: AN EFFECTIVE TOOL FOR DRUG ESTIMATION

    About Authors:
    Bhawana Kapoor*1, Vishnukant Rai2, Sonu Sharma3
    1Seth G.L. Bihani S.D. College of Technical Education, Sriganganagar, Rajasthan, India
    2Shri Ramnath Singh College of Pharmacy, Gormi, Bhind, M.P., India
    3NIMS Institute of Pharmacy, Jaipur, Rajasthan, India

    ABSTRACT
    Chromatography, although primarily a separation technique, is mostly employed in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. The reasons for this include the simplicity, versatility, and scope of the reversed-phase method as it is able to handle compounds of a diverse polarity and molecular mass. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development.

  • Quantitative Estimation of Piperine in Ayurvedic Formulations by HPTLC

    About Authors:
    Ujjwal S. Yeotkar*, Mahendra Nimbhorkar, Tushar A. Deshmukh, Vijay R. Patil
    Department of Pharmacognosy,
    Tapi Valley Education Society’s,
    Hon’ble, Loksevak Madhukarrao Chaudhari College of Pharmacy,
    North Maharashtra University,
    Faizpur- 425 503,
    Maharashtra, India.

    ABSTRACT
    Churna are important group of formulation used by Ayurvedic physicians to treat various types of diasease. Trikatu and Pimpali churna, as per Ayurvedic literature is used for the treatment of resperatory disorders. In the present study,an attempt has been made to develop a HPTLC  method of quantitative estimation of marker compound, piperine in laboratory prepared authentic formulation and a marketed formulation of Trikatu and Pimpali churna. The stationary phase used was precoated silica gel G 60 F 254 plates. The mobile phase containing n- Hexane-ethyl acetate, (5:5 v/v ), was used to separate the spot of Piperine. Plates were developed to a distance of 8 cm at room temperature. Spectrodensitometric scanning was performed by TLC scanner III (CAMAG) in absorbance mode at the wavelength of 339nm. The  Rf  values of  piperine was 0.44± 0.02. The method was validated in terms of Linearity, Accuracy and  Precision. The linearity curve found to be linear in between 200–900 ng/spot. The limit of Detection (LOD) and limit of Quantification (LOQ) were found to be  1 ng and  3 ng respectively. The mean results from % Recovery was found to be, laboratory formulation contain 85.13% and 83.47% while the commercial formulation shows 96.65%, and 90.32% respectively. The proposed method can be used to determine Piperine from Ayurvedic formulations.

  • Introduction to new chromatography technique - UPLC

    About Authors:
    Snehal V.Chopade, Dr.V.R.Patil - Principle
    TVES College of pharmacy,
    Faizpur accredited by North Maharashtra University,
    Jalgaon

    Abstract:
    UPLC can be regarded as new invention for liquid chromatography. UPLC refers to Ultra Performance Liquid Chromatography. UPLC brings dramatic improvements in sensitivity, resolution and speed of analysis can be calculated. It has instrumentation that operates at high pressure than that used in HPLC & in this system uses fine particles(less than 2.5µm) & mobile phases at high linear velocities decreases the length of column, reduces solvent consumtion & saves time.
    This review introduces the theory of UPLC, and it can summarizes some of the most recent work in the field.

  • TROUBLESHOOTING IN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

    About Authors:
    Ipshita Chattopadhyaya, Ekta Malhotra
    Department of Pharmacy,
    Jaipur National University,
    Jaipur, India

    ABSTRACT
    (1)The developments in column packing technology and suitable equipment paved the way for what is now called High Performance or High Pressure Liquid Chromatography (HPLC). The new technique provided much higher resolution, more accurate quantitative results, as well as shorter analysis times in comparison to the earlier techniques .During the sixties, new theoretical insights accompanied by important. Since its introduction, HPLC has evolved into an indispensable tool in many analytical laboratories and is applied to diverse analytical problems. Actually, HPLC refers to a number of separation techniques that use a liquid mobile phase, or eluent. Troubleshooting HPLC instrumentation and separations require a fundamental understanding of how the instrument functions and how the separation works. (2)This article provides a practical guide to common HPLC problems, along with more in-depth information to help the reader understand the relationships between the observed symptoms and the underlying causes. The practical approach presented here is meant to serve as both a troubleshooting guide and an HPLC learning tool.

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  • ESTIMATION OF SPIRONOLACTONE AND TORSEMIDE IN COMBINED TABLET DOSAGE FORM USING BY MULTICOMPONENT MODE OF ANALYSIS

    About Authors:
    Kapil Sharma1*, Subhash Gupta 2, Yogesh Sharma1
    1 Yaresun Pharmaceuticals Pvt. Ltd.Jaipur - 302006, Rajasthan, India.
    2 Oasis test house ltd.jaipur-302006,
    Rajasthan, India.

    SPECTROPHOTOMETRIC SIMULTANEOUS ESTIMATION OF SPIRONOLACTONE AND TORSEMIDE IN COMBINED TABLET DOSAGE FORM USING BY MULTICOMPONENT MODE OF ANALYSIS

    ABSTRACT
    A method for simultaneous estimation of torsemide (TRS) and spironolactone (SPL) in combined tablet dosage form has been developed. The method employs the application of multicomponent mode of analysis. This method utilize 50 % v/v methanol in distilled water. TRS show maximum absorbance at a wavelength of 288 nm and SPL at 238 nm. Where the linearity ranges for TRS and SPL were 1-5µg/ml and 5-25 µg/ml respectively. The procedure was successfully applied for the simultaneous determination of both drugs in laboratory prepared mixture and in market available tablet dosage form. The accuracy of the method was assessed by recovery studies and was found to be 98.15±0.64 and 100.27±0.15 for TRS and SPL respectively. Results of the analysis were validated statistically so that it can be used for routine analysis of TRS and SPL in combined tablet dosage form.

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  • VALIDATION OF ANALYTICAL PROCEDURES: A COMPARISON OF ICH Vs PHARMACOPOEIA Vs FDA

    About Authors:
    Kataria Sahil,Middha Akanksha, Sandhu Premjeet
    Seth G. L. Bihani S.D. College of Technical Education,
    Institute of Pharmaceutical Sciences and Drug Research,
    Sri Ganganagar, Rajasthan, INDIA

    ABSTRACT
    Method validation is the process used to confirm that the analytical procedure employed for a specific test is suitable for its intended use. Results from method validation can be used to judge the quality, reliability, and consistency of analytical results; it is an integral part of any good analytical practice. When extended to an analytical procedure, depending upon the application, it means that a method works reproducibly, when carried out by same or different persons, in same or different laboratories, using different  reagents,  different  equipment etc.  In  this  review  article  we  discussed  about  the  strategy  and  importance  of  validation  of  analytical  methods.

  • QSAR Analysis on Some Novel 4-Quinolylhydrazone Derivatives as Anti- Tubercular agent

    About Authors:
    Dr. A. K. Pathak, Parul Sengar, Kamlesh Kumar*
    Department of Pharmacy, Barkatullah University
    Bhopal, M. P.

    Abstract
    A Quantitative Structure Activity Relationship study on a Series of 40 molecules of (4-Quinolylhydrazone compounds) with anti-tubercular activity analogues was made using combination of various physicochemical descriptors (Thermodynamic, electronic and spatial). Several statistical expressions for 2D QSAR & 3D QSAR were developed using stepwise partial least square (PLS) regression analysis and K-Nearest neighboring molecular field analysis (K-NNMFA) respectively. The studies on 2D-QSAR, suggested the four descriptors T_C_C_2, T_C_N_4, Surface area excluding P & S, SsCH3E-index, and H donor count were common and highly contributed the activity. 2DQSAR model developed using partial least square regression approach. Negative logarithmic value of (MIC) was taken as dependent variable and selected discriptors were taken as independent varable. The analysis resulted in the following 2D-equation suggest that, MIC50 = [+0.1587 T_C_C_2- 0.0593 SsCH3E-index- 0.1395 T_C_N_4+ 0.3888 H-Donor Count -3.1556], n =26; Degree of freedom = 22; r2 = 0.75; q2 = 0.67; F test = 22.63;  r2 se = 0.20 ; q2 se = 0.23; pred_r2 = 0.46 ; pred_r2 se = 0.24, a H-donor group at Ar, is important for guiding the design of a new molecule. 3DQSAR model developed using K-nearest neighbour method (training set =33 and test set = 7). The best model derived by the method have q2 = 0.51, q2_se = 0.25, Predr2 = 0.71, pred_r2se = 0.16, n=33, k Nearest Neighbor is 2, Degree of freedom =28. The steric and electrostatic descriptors at the grid points, E_916 (0.1865, 0.3006); S_591 (-0.2851, -0.2580); S_1027 (-0.3273, -0.3090); S926 (30.000, 30.000) plays important role for design of new molecule. 3DQSAR analysis of series of 4-Quinolylhydrazone compounds informed that electropositive and less bulky group increases the biological activity.

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