Ritobrata Rudra*1, Sudipta Ganguly2, Manabendra Dhua3, Binny sikdar4
1Department of pharmaceutical chemistry, Gupta College of Technological Sciences, Asansol.
2Department of Quality control, Stadmed pharmaceutical pvt ltd, Dumdum, Kolkata.
3Department of pharmaceutical chemistry, Gupta College of Technological Sciences, Asansol, W.B.
4North Bengal University, silliguri, W.B.
Developing a single analytical method for estimation of single drug from its dosage form is a very challenging task. A simple, rapid, precise, and reliable reverse phase HPLC method was developed for the separation and estimation of Terazosin HCl in pharmaceutical dosage forms. The estimation was carried out using Inertsil ODS-C18(150 mm × 4.6 mm, 5 μm) column; mobile phase consisting of water, acetonitrile, triethylamine, and pH 6.4 adjusted by orthophsphoric acid; the flow rate of 1 mL/min and ultraviolet detection at 245 nm. Run time of this drug is 2.3 minutes. The method was validated as a final verification of method development with respect to precision, linearity, accuracy, ruggedness, and robustness. The validated method was successfully applied to the commercially available pharmaceutical dosage form, yielding very good and reproducible result.