Andhra Pradesh

GLANZMANN’S THROMBASTHENIA : A RARE BLEEDING DISORDER

ABOUT AUTHORS
SRI HARSHA AYYAGARI.*HARI PRIYA MULLAMURI, SOWMYA  BYREDDY,   DURGA PRASAD T. S.
Sri Padmavathi School of pharmacy, Jawaharlal Nehru Technological University, Anantapuram, Andhra Pradesh, India.

ABSTRACT
Glanzmann's Thrombasthenia (GT) is a rare, autosomal recessive bleeding disorder characterized by prolonged bleeding time and impairment in aggregation of platelets and impairment in clot retraction. The physiological defect includes impairment of glycoprotein receptor’s (GPIIb/IIIa complex) present on platelet membrane which mediates platelet aggregation through fibrinogen binding. Purpose of this study is to get  all of us know about this rare condition. A 16-year old female patient presented with repeated episodes of menstrual bleeding since 4years. She was a k/c/o Glanzmann's thrombasthenia. She was treated with antifibrinolytics, IV fluids and then discharged. Early diagnosis and proper supportive care are the measures for prognosis of Glanzmann’s thrombasthenia.

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VALIDATED STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DROTAVERIN.HCL & ACECLOFENAC IN BULK AND FORMULATION

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ABOUT AUTHORS
A. PADMA1*, D. GIRIJA2, A. THIRUPATHIREDDY3, B. VENKATESWARAREDDY4
1Department of Pharmaceutical Analysis, Sankar Reddy Institute of Pharmaceutical sciences, Salakalveedu(v), Bestavaripeta(M), Prakasam (Dist) Andhra Pradesh, India
2Department of Pharmaceutical Analysis, Sankar Reddy Institute of Pharmaceutical sciences, Salakalveedu(v), Bestavaripeta(M), Prakasam (Dist) Andhra Pradesh, India
3Department of Pharmaceutics, Sankar Reddy Institute of Pharmaceutical sciences, Salakalveedu(v), Bestavaripeta(M),Prakasam(Dist) Andhra Pradesh, India
4Department of Pharmaceutics, Sankar Reddy Institute of Pharmaceutical sciences, Salakalveedu(v), Bestavaripeta(M), Prakasam(Dist) Andhra Pradesh, India

ABSTRACT
The present research work deals with the development  and validation of  stability indicating RP-HPLC method for the simultaneous estimation of Aceclofenac and Drotaverin.HCl in bulk and formulation. Chromatographic separation was achieved on ODS HG-5 RP   C18(250 X 4.6 mm, 5 µm) using the mobile phase consisting of Acetonitrile and1% triethylamine (TEA) in the ratio of 90:10 pH  2.16 adjusted with Ortho phosphoric acid. The mobile phase was pumped at a flow rate of 1.0 mL/min and UV detection was observed  at 224 nm. The retention time was found to be 3.57 min for Aceclofenac and 6.15 min for Drotaverin.HCl. The linearity concentrations are obtained from in the range of 30-70 µg/ml for Aceclofenac and 24-56 µg/ml for Drotaverin.HCl respectively with correlation coefficient was 0.999 and 0.998. The proposed method was found to be simple, accurate, precise and reproducible and developed method can be suggest to routine quality control analysis for simultaneous estimation of Aceclofenac and Drotaverin.HCl in pharmaceutical dosage forms.

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