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Development and Validation of Analytical Methods for Simultaneous Estimation of Amitriptyline Hydrochloride and Methylcobalamin in their Tablet Dosage Form by UV Spectrophotometric Method

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JULY 2015 ARTICLE LIST >>

PharmaTutor (July- 2015)

 

Print-ISSN: 2394 - 6679
e-ISSN: 2347 - 7881
(Volume 3, Issue 7)

 

Received On: 12/03/2015; Accepted On: 26/03/2015; Published On: 01/07/2015

 

AUTHORS: Chetna V. Karchaliya*, Parula B. Patel
Department of Quality Assurance,
S. J. Thakkar Pharmacy College, Rajkot, Gujarat, India
*sonichetna158@gmail.com

 

ABSTRACT: The simple, accurate and precise Absorption Correction Method has been developed for the simultaneous estimation of Amitriptyline hydrochloride and Methylcobalamin in combined tablet dosage form. The method utilizes distilled water as solvent and λmax of Amitriptyline hydrochloride and Methylcobalamin selected for analysis were found to be 239 nm and 351 nm respectively. The method was validated as per International Conference on Harmonization (ICH) guidelines. The Linearity range lies between 20-60 µg/ml (R2 0.9998) for Amitrityline hydrochloride and 3-9 µg/ml (R2 0.9990) for methylcobalamin. The accuracy and precision were determined and found to comply with ICH guidelines. The method showed good reproducibility and recovery with %RSD in desired range. The proposed method can be applied for routine analysis of both drugs.

 

How to cite this article: CV Karchaliya, PB Patel; Development and Validation of Analytical Methods for Simultaneous Estimation of Amitriptyline Hydrochloride and Methylcobalamin in their Tablet Dosage Form by UV Spectrophotometric Method; PharmaTutor; 2015; 3(7); 46-50

 

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