Difference Spectroscopic Method for the Estimation of Acebutolol Hydrochloride in Bulk and In its Formulation
PharmaTutor (February- 2015)
e-ISSN: 2347 - 7881
(Volume 3, Issue 2)
Received On: 21/11/2014; Accepted On: 28/11/2014; Published On: 01/02/2015
AUTHORS: Jadhav Santosh*, Mali Audumbar, Pawar Seemarani, Kharat Rekha, Tamboli Ashpak
Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade,
Sangola-413307, Solapur, Maharashtra, India.
ABSTRACT: A simple, precise and accurate difference spectroscopic method has been developed for the estimation of Acebutolol Hydrochloride in bulk drug form by difference spectrophotometric method. Acebutolol Hydrochloride has exhibited maximum absorbance at about 233nm and 234nm in acidic and basic solution respectively. Beer’s law was obeyed in the concentration range of 2-10 µg/ml in both the cases. The regression of coefficient was found to be r2=0.9992. The LOD and LOQ value were found to be 0.2670ppm and 0.8091ppm respectively. The proposed method was successfully applied for the determination of Acebutolol Hydrochloride in bulk drug. As per ICH guidelines the result of the analysis were validated statistically and were found to be satisfactory.
How to cite this article: S Jadhav, A Mali, S Pawar, R Kharat, A Tamboli; Difference Spectroscopic Method for the Estimation of Acebutolol Hydrochloride in Bulk and In its Formulation; PharmaTutor; 2015; 3(2); 53-57