Pharma Analysis

Q.5. (a)Write principle difference between conventional chromatography and HPLC.
Discuss pumping system, stationary phases and detectors with applications.
Ans.5. (a) Difference between Conventional chromatography and HPLC

Q.4. (b) Recognition of molecular ion and its role in structure determination

Q.4.(a) Basic fragmentation types, rules and factors influencing it.

Ans.4.(a) There are 3 basic types of fragmentation:

Q.3.(c) Spectrum editing DEPT spectra and applications

Ans.3. (c)DEPT

Q.3. (b) Simplification of complex proton NMR spectrum

Q.3.(a)Spin-spin splitting and factors influencing coupling constant

M.Pharma

First semester examination, 2008-2009

Modern analytical techniques

Dr A.P.J. Abdul Kalam Technical University

Q1. Attempt any two parts of the following:

(a)  Define and exemplify chromophores. Discuss their interaction with UV- visible radiation and illustrate their use in structural analysis of organic compounds.

(b) Cotton effect curves and octant rule.How would you relate stereo chemical features of a compound with them?

(c)  Write a note on ATR and its application in specific cases.

Q.2. (c) Discuss ELISA assay for antibody by indirect methods and antigen by sandwich method. Enzyme Linked ImmunosorbentAssay (ELISA)

Ans.2. (c)The steps of the general, "indirect," ELISA for determining serum antibodyconcentrations are:

Q.2. (b)How would you identify various functional groups by IR spectroscopy provide supporting examples.

Ans.2. (b)Identify various functional groups by IR spectroscopy: