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A REVIEW ON SINGLE USE DISPOSABLE TECHNOLOGY FOR RECOMBINANT PROTEIN MANUFACTURING

ABOUT AUTHOR
Madhusudan P Dabhole
Group Manager – BioProcess,
Richcore Life Sciences Ltd, Bangalore, Karnataka, India
madhav888@rediffmail.com

ABSTRACT
The manufacturing of recombinant products by fermentation and purification in stainless steel vessels has seen the transition from small scale to large scale and further to single use disposable technology. The requirement to develop and modulate the process has arisen from the cost and manufacturers need to move the facility on mobile platforms. The review describes the strategies and considerations for Single Use Disposable Technology. Recombinant proteins are widely used for treatment of various diseases and disorders. Single Use Disposable Technology makes it promising to produce and formulate these proteins from bench scale to commercial level in a shorter span of time so that it can reach the physician and patients.


COMPARATIVE EVALUATION OF PURIFICATION METHODS FOR PRODUCTION OF POLYPEPTIDE ANTIBIOTICS – “POLYMYXIN B” AND “CEREXIN A” FROM BACILLUS SPECIES

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ABOUT AUTHORS:
Pratyush Kumar Das1, Shilpa Das1, Debasish Sahoo2, Jikasmita Dalei2, V.Madhav Rao2, Sunakar Nayak2, Swadhin Palo3
1Centre of Biotechnology, Siksha O Anusandhan University, Bhubaneswar, Odisha, India.
2Nitza Biologicals (P.) Ltd.Chandra Towers, Near Fortune Honda Showroom, Neredmet 'X' Road, Secundrabad, Andhra Pradesh, India.
3Roland Institute of Pharmaceutical Sciences, Berhampur, Odisha, India.
onlypratyush11@gmail.com

ABSTRACT:
Polymyxin B and Cerexin A are two polypeptide antibiotics, the first one discovered and incorporated quite earlier and the later one has still not been used in clinical trials for its high cytotoxic nature. Although Polymyxin was discovered very earlier but in the mid-way for some time it had lost its importance and was not used frequently due to its narrow spectra of action that only acts on gram negative microbes and because of its toxicity level. But with several new resistant gram negative microbes coming into the limelight responsible for causing many infections, Polymyxin B (the least toxic of all Polymyxins) has again been started to be used in pharmaceutical formulations and drugs. In this project, both Bacillus polymyxa and Bacillus cereus responsible for production of Polymyxin B and Cerexin A respectively were isolated from the rhizosphere of grass and cultured in the lab. They were confirmed by biochemical tests and then used to produce the corresponding antibiotics by submerged fermentation. The crude antibiotic thus obtained were purified by various methods like adsorption through activated charcoal, acetone precipitation, dialysis, Ion Exchange and Sephadex column chromatography and the results were compared to find the best possible way to purify the antibiotics keeping in mind that they show the maximum activity as possible on a lab scale. Further work on Cerexin A was not possible due to the unavailability of its standard solution. Work was carried out for quantitative estimation of purified and crude Polymyxin B by performing spectrophotometric assay against standard polymyxin.


DNA MICROSATELLITES: A REVIEW

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About Authors:
Shikha Jain*, Ranjana Joshi, Kirti Jatwa, Avnish Sharma, S.C. Mahajan
Department of Pharmaceutics,
Mahakal Institite of Pharmaceutical Studies,
Behind Air strip, Datana, Dewas Road, Ujjain, M.P.
jain.shikhapharma@gmail.com

Abstract
Microsatellites consist of tandemly repeated sequence, no more than 6 bases long. They are scattered throughout most eukaryotic genomes. The major characteristic that makes microsatellites as useful and powerful genetic tool is the extensive length polymorphism that first of all reflects allelic variation in the number of the tandemly arranged perfect repeats. Microsatellite are generally considered as the most powerful genetic marker.
A genetic marker is a gene or DNA sequence with a known location on a chromosome that can be used to identify individuals or species. Genetic marker that reveal polymorphisms at the DNA level are called molecular marker.
Molecular markers are called as DNA markers, which should be thought of as signs along the DNA trail that pinpoint the location of desirable genetic traits or indicate specific genetic differences.They are responsible for various neurological diseases and hence the same cause can now be utilized for the early detection of various diseases, such as, Schizophrenia, Bipolar Disorder and Congenital generalized Hypertrichosis . These agents are widely used for forensic identification and relatedness testing, and are predominant genetic markers in this area of application.


AMPHIPHILIC JANUS LIKE PARTICLES FOR BIOMEDICAL APPLICATION

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ABOUT AUTHOR:
*1Patitapabana Parida, 1Bibhukalyan P Nayak, 2Subash Chandra Mishra
1Department of Biotechnology and Medical Engineering,
2Department of Metallurgy & Materials Engineering
National Institute of Technology Rourkela, Odisha, India
*paridap@nitrkl.ac.in

ABSTRACT
Nano structure possesses high surface area that the functional groups can be able to expose their reactive capacity due to high surface energy. So as to consider their size of the amphiphilic nanoparticle dual-surface structure containing body called as janus particle introduced since about twenty years in industry with purpose of drug delivery and diagnostic purpose in the field of paramedical areas. Janus bodies can carry both lipophilic and hydrophilic groups on its surface. Nano-sized morphology could play multiple roles in cancer treatment including diagnosis, biomedical imaging, tissue engineering and drug delivery.


BIOTECHNOLOGY FOR A BETTER WORLD

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ABOUT AUTHORS:
Nirav.R. Soni
M.Pharm, A-One Pharmacy College,
Anasan, Ahmedabad-382330, India
nirav_sonic@yahoo.com

ABSTRACT:
Pharmaceutical Biotechnology is not a source of energy, but a scientific method that provides tools to produce energy based entirely on modern biotechnological techniques, as to date encompass a wider range of altogether newer medicinal compounds, e.g., antibiotics, vaccines development and monoclonal antibodies (MABs) that may now be produced commercially using well-defined, optimized and improved fermentative methodologies. In fact, genetic engineering has brought in a sea change by virtue of the directed construction of microorganisms resulting in a plethora of newer life-saving drugs. Focus on a variety of  Research areas including health/medicine, food science, environmental science and agri- science and also bioenergy development.


TILLING: VERSATILE REVERSE GENETIC TOOL

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About Authors:
Navgeet1, Balraj Singh Gill2, Arvind Negi3*, Shashi Shekhar Anand2
1Institute of Himalayan Bioresource and Technology (CSIR-IHBT), Palampur (HP)
2Centre for Biosciences Central University of Punjab, Bathinda (Punjab)
3Centre for Chemical and Pharmaceutical Sciences, Central University of Punjab, Bathinda (Punjab)
*arvindnegi2301@gmail.com, arvindnegicup@gmail.com

Abstract:
Recent years have been witnessedfor exhaustive genome sequencing, nourishing a cause to track the mutation and its consequences at the phenotypic level. Butreliabilityof these genomic studiesis the critical issuewhich is still unaddressed and not properly understood. Moreover via TILLING techniques (reverse genetic tool), can be valuable in evaluation of these studies to much extent. Usually TILLING account on a specific gene mutation in order to observe the extent of the functionality of that particular gene at morphological level. This review compiles the literature pertaining to the art of tilling in the evaluation of genomic studies and majorly in concern of functionalgenomics. Moreover this review also covers the example of the most common species so as to buildbetter understanding of the concept behind this technique of reversegeneticsand can be fruitfully applied in extractingthe ethano-botanical and therapeuticeffect of various medicinal plants.


A NEW PROMISE: NEURAL TISSUE ENGINEERING USING NANOTECHNOLOGY

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About Authors:
1*
Neeraj Kumar Lohani, 2Vachaspati Mishra, 3Divakar Joshi
1,2
Department of Biotechnology, Institute of Biomedical Education and Research, Mangalayatan University, Beswan, Aligarh-Uttar Pradesh, india,
3
MBPG College Haldwani Nainital Kumaun University Nainital Uttarakhand.
neerajlohani06@gmail.com

Abstract
The interdisciplinary approach with nanotechnology and animal tissue culture technique is going to revolutionize biomedical science in the next fifty years. Nanotechnology along  with regulated animal tissue culture, makestissue engineering a realization  based on the creation of new tissues in vitro followed by surgical placement in the body or the stimulation of normal repair in situ using bio-artificial constructs or implants of living cells introduced in or near the area of damage at nano level.It makes use of artificially stimulated cell proliferation by using suitable nano-material based scaffolds and growth factors. Nanotechnology can be successfully used to create a tissue or organ that can take the place of one that is terminally diseased, such as an eye, ear, heart, or joint. Implantable prosthetic devices and nano scaffolds are used for growing of artificial organs. The key components of tissue engineering with nanotechnology include: cells, scaffolds, signals and bioreactors. Scaffolds are produced by electro-spinning technique.The scaffold acts as an interim synthetic extra cellular matrix (ECM) that cells interact with prior to forming a new tissue. 


DRUG DESIGNING : A REVIEW

ABOUT AUTHOR:
Muhammed Mujahed
Master’s of Science in Biotechnology.
SRTM University.
mujubiotech2011@rediffmail.com

INTRODUCTION:
Drug design is an integrated developing discipline which portends an era of ‘tailored drug’. It involves the study of effects of biologically active compounds on the basis of molecular interactions in terms of molecular structure or its physico-chemical properties involved. It studies the processes by which the drug produce their effects, how they react with the protoplasm to elicit a particular pharmacological effect or response how they are modified or detoxified, metabolized or eliminated by the organism.

Disposition of drugs in individual region of biosystems is one of the main factors determining the place , mode and intensity of their action . The biological activity may be “positive” as in drug design or “negative” as in toxicology. Thus drug design involves either total innovation of lead  or an optimization of already available lead. These concepts are the building stones up on which the edifice of drug design is built up.

The drug is most commonly an organicsmall  molecule that activates or inhibits the function of a biomolecule such as a protein, which in turn results in a therapeutic benefit to the patient. In the most basic sense, drug design involves the design of small molecules that are complementary in shape and charge to the biomolecular target with which they interact and therefore will bind to it. Drug design frequently but not necessarily relies on computer modeling techniques. This type of modeling is often referred to as computer-aided drug design. Finally, drug design that relies on the knowledge of the three-dimensional structure of the biomolecular target is known as structure-based drug design.


EVIDENCE-BASED MEDICINE TO PERSONALIZED MEDICINE – A PROSPECTIVE

About Author:
Pritish Dash
Institute of Bioinformatics and Applied Biotechnology (IBAB)
Bangalore, India
pr4evr@gmail.com

Abstract:
Evidence-based medicine (EBM) is the process of systematically reviewing, appraising and using clinical research findings to aid the delivery of optimum clinical care to patients. It is a method of healthcare decision-making that intends to combine the most reliable scientific information with individual expertise and patient preferences in order to offer the optimal diagnostic and therapeutic option for the patient. On the other hand, pharmacogenomics is a whole genome application that examines the single gene interactions with drugs. In recent years, the term personalized medicine has been introduced to represent an approach considering differences among individual patients. In modern medicine, the most important sources of evidence are clinical trials using epidemiological methods, and molecular biological and genetic methods characterizing individual patients.
This paper tries to review the rapid transformation of modern medicine from the ‘evidence-based medicine’ to ‘personalized and genomic medicine’.


EDIBLE VACCINES

About Author:
K.Kranthi Kumar
SKU College of Pharmaceutical Sciences,
S.k.univrsity, Anantapur
kranthikumarkotta@gmail.com

Abstract
Molecular farming officially known as transgenic non-food GM plant pharming and biopharming, is a type of genetic modification used in farming involving the use of plants, and potentially also animals, as the means to produce compounds of therapeutic value. The idea is to use such crops as biological factories to generate drugs difficult or expensive to produce in any other way. The issue of genetically modified crops has been around for a number of years and continues to be a controversial subject.