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Biotechnology Articles
PREPARATION OF ANTI HUMAN IgG – HRP CONJUGATE BY WESTERN BLOT TECHNIQUE
About Authors
1M Prasad Naidu, 2T Madhu Chaithanya, 3N Mallikarjun Rao, 4Muneer Bhanu , 5Dr Madhu Sudan Reddy
1Medical Biochemistry, NMCH
2Medical Pharmacology, NMCH
3Biochmeistry, NMCH
4Biotechnology, NMCH
5MD Pharmacolgy, NMCH
amarnageshkumar@gmail.com
Introduction
Conjugation of enzymes to antibodies involves the formation of a stable, covalent linkage between an enzyme [e.g., horseradish peroxidase (HRPO), urease, or alkaline phosphatase] and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site nor the active site of the enzyme is functionally altered. The chemistry of cross-linking HRPO or urease to immunoaffinity purified monoclonal or polyclonal antibodies (IgG) is presented in. The chemistry of cross-linking alkaline phosphatase to antibodies is presented in. The enzyme most commonly used in the immunoreagent (the antibody enzyme conjugate) preparation is horseradish peroxides. This enzyme is cheap and can be attached to the immunoreagent by a variety of methods. Moreover many chromogenic substrates for it are also available.
IN SILICO DRUG DOCKING OF PHYTO INHIBITORS AGAINST TRIOSEPHOSPHATE ISOMERASE IN PLASMODIUM FALCIPARUM
ABOUT AUTHORS:
Pramod Shinde, Vijay s. Savakare
Department of Bioinformatics, Guru Nanak Khalsa College,
Matunga, Mumbai-19, India.
vssavakare@gmail.com
ABSTRACT:
Malaria caused by the parasite Plasmodium falciparum is a major public health concern. The parasite lacks a functional tricarboxylic acid cycle (TCA), making glycolysis its sole energy source. One such enzyme is triose phosphate isomerase. This catalyses the isomerization of D-Glyceraldehyde 3 phosphate to dihydroxy acetone phosphate. An attempt was made to identify the potential phyto inhibitors and inhibit the enzyme as well as to modify their side chain to impure the binding efficiently. Here, two datasets are made such as training set and testing set,in which first, is training set, contain 20 known inhibitors against Triosephosphate Isomerase and second is testing set which contain 7 phyto-inhibitors to be tested. Autodock Vina, a docking tool, is used for molecular docking that utilizes information on conformational variability from ensembles of experimental receptor structure ofTriosephosphate Isomerase. We show that experimentally determined binding orientations and computed energies of known Ligands can be reproduced accurately. It was reported that the presence of phosphate groups in a ligand confers better stable docking.
REVIEW: PROCESS VALIDATION IN BIOTECHNOLOGY
ABOUT AUTHORS
Abhijeet Welankiwar*, Sushant Tope
Govt. college of Pharmacy Kathora naka
Amravati (Maharashtra) 444604.
*abhi123welankiwar@gmail.com
ABSTRACT:
The validation is a Fundamental segment that supports to a commitment of company towards quality assurance. It also assures that product meets its predetermined quality specification and quality characteristics. Validation of individual step of manufacturing is called as process validation. This Article concerns with the validation of biotechnological process. It is generally complex than validation of traditional synthetic or naturally occurring small molecules of drugs. Its level of complexity depends upon type of biotechnological products. The validation of biotechnological process has 3 Basic aspects they are Risk factors that are needed to be addressed, analytical tools necessary for validation and validation of unit operations.
DISCUSSION ON BIOREMEDIATION AND ITS APPLICATIONS:AN OVERVIEW
About Authors:
Lohithasu Duppala*1, Madhu priya Damuluri,1 Anilkumar vadda2
1GITAM Institute of Pharmacy, GITAM University, visakhapatnam, Andhra Pradesh, India-530045.
2AVANTHI Institute of pharmaceutical sciences, pharmacology, visakhapatnam, Andhra Pradesh,India-530045.
*lohithasupharma@gmail.com
ABSTRACT:
The environmental biotechnology employs the application of genetic engineering to improve the efficiency and cost which is essential to the future wide spread exploitation of microorganisms to reduce the environmental burden of toxic substances. Now a days it can be achieved by bioremediation, is the process by using microorganism metabolism to remove pollutants and organic substances. It may be employed to attack specific soil contaminants, such as degradation chlorinated hydrocarbons by bacteria.
AN OVERVIEW ON DNA MICROARRAY TECHNOLOGY
About Author:
Krishna Bhatt
Department of Pharmacy, B.N. Girls’ College of Pharmacy,
(Udaipur, INDIA.)
krishna30.bhatt@yahoo.com
Abstract:
The vast amount of information available through the human genome project is going to have a major impact on medical science. However, the mere sequence information of the whole genome does not answer all our questions. What is required at this stage is a complete understanding of the function of genes and other parts of the genome so as to uncover how sets of genes and their products work together in normal and diseased conditions. One major requirement for these studies is the development of high-throughput technologies. DNA microarrays are some of the most powerful and versatile tools available, and there are several applications of microarray technology e.g. cancer.
Computational Analysis of HCV Entry Inhibitors for Hepatitis C Treatment – A Molecular Docking Approach
About Author:
Karthika M
Postgraduate in Bioinformatics
Bharathiar University
m.karthika1@gmail.com
ABSTRACT
Hepatitis C virus (HCV) is a major human pathogen associated with life-threatening liver disease, affect 170 million people worldwide. Hepatitis C virus (HCV) envelope proteins mediate the entry of virus into cells by binding to cellular receptors, resulting in fusion of the viral membrane with the host cell membrane and permitting the viral genome to enter the cytoplasm. Entry inhibitors would inhibit the viral entry itself.
A STUDY ON THE ECOFRIENDLY DYES EXTRACTED FROM THREE DIFFERENT SPECIES OF Curcuma L.
About Authors:
*Nikhala Shree
Department of plant biology and plant biotechnology Women’s christian college
An Autonomous Institution – Affiliated To The University Of Madras
Chennai-600 006
*nikhala.shree@gmail.com
1. Introduction:
Natural dyes are known for their use in colouring of food substrate, leather, wood as well as natural fibers like wool, silk, cotton and flax as major areas of application since ancient times. Natural dyes may have a wide range of shades, and can be obtained from various parts of plants including roots, bark, leaves, flowers, and fruit (Allen 1971). Since the advent of widely available and cheaper synthetic dyes in 1856 having moderate to excellent colour fastness properties, the use of natural dyes having poor to moderate wash and light fastness has declined to a great extent.
Biosensor for HIV Diagnosis at All Stages of Infection- A Nano Idea
About Authors:
Dhanya V C
Kasturba medical college, Manipal University
Indian Veterinary Research Institute
dhanya285@gmail.com
INTRODUCTION:
A biosensor is a device that uses specific biochemical reactions mediated by isolated enzymes, immunosystems, tissues, organelles or whole cells to detect chemical compounds usually by electrical, thermal or optical signals (1). Or in other words, Sensor that integrates a biological element with a physiochemical transducer to produce an electronic signal proportional to a single analyte which is then conveyed to a detector.
EFFECT OF LYOPHILIZATION AND CRYOPRESERVATION ON PLANT LEAVES OF TERMINALIA ARJUNA TERMINALIA CATAPPA, TERMINALIA CHEBULA, JATROPHA GOSSYPIFOLIA, JATROPHA CURCAS
About Authors:
1Hardik R. Patel*, 2Upanita C. Patel
1Industrial biotechnologist, 2Microbiologist
Anand, Gujarat, India.
*hardikigbt@gmail.com
INTRODUCTION:
Cryopreservation and lyophilization of plant germplasm has obvious advantages over in vitro storage in term of space saving and improved phytosanitation. We compared cryopreserved and lyophilized leaf as sources for genomic DNA isolation by CTAB protocol and PVP protocol.Our results showed that cryopreservation of leaf tissue yielded high molecular weight genomic DNA. The DNA was suitable for restriction-enzyme digestion and as a template for polymerase chain reaction (PCR) amplification. While these results rule out cryopreserved tissue as a source for DNA isolation, the ability to freeze-dry, powder, and efficiently store voluminous tissue samples for later use in DNA and protein isolation could be of great benefit to laboratories involved in molecular genetics and molecular biology.
EFFECT OF CRYOPRESERVATION, LYOPHILIZATION ON DNA EXTRACTION PROTOCOL OF ACACIA ARABICA, ACACIA SINUATA, PROSOPIS SPICIGERA, ADENANTHERA PAVONINA AND ACACIA AURICULIFORMIS
About Authors:
Hardik R. Patel
Industrial Biotechnology from Sardar Patel University,
Gujarat, India.
hardikigbt@gmail.com
ABSTRACT
Cryopreservation and lyophilization of plant germplasm has obvious advantages over in vitro storage in term of space saving and improved phytosanitation. We compared cryopreserved and lyophilized leaf as sources for genomic DNA isolation by CTAB protocol and PVP protocol.Our results showed that cryopreservation of leaf tissue yielded high molecular weight genomic DNA. The DNA was suitable for restriction-enzyme digestion and as a template for polymerase chain reaction (PCR) amplification. While these results rule out cryopreserved tissue as a source for DNA isolation, the ability to freeze-dry, powder, and efficiently store voluminous tissue samples for later use in DNA and protein isolation could be of great benefit to laboratories involved in molecular genetics and molecular biology.
