You are herePHARMACODYNAMIC INTERACTION OF FENUGREEK LEAVES EXTRACT WITH FLUVASTATIN IN DIABETES INDUCED VASCULAR DYSFUNCTION

PHARMACODYNAMIC INTERACTION OF FENUGREEK LEAVES EXTRACT WITH FLUVASTATIN IN DIABETES INDUCED VASCULAR DYSFUNCTION


EXPERIMENTAL ANIMAL MODELS:
Isolated Rat Aortic ring preparation:

The rat aortic preparations were isolated, mounted in a temperature controlled tissue/organ bath and suspended in K-H solution that was aerated with carbogen gas so as to maintain the isolated tissues in a physiological state i.e. in a viable state during the experimental period.Thus using the tissue /organ bath the necessary physiological warmth is provided to the tissue even after  isolation from the animal while the K-H solution is used to provide the essential medium containing the various ions, buffer, energy source etc. but also to supply oxygen requirements to the isolated tissue.

RESULTS: 
Table-1.Effect of FLEt and its combination with Oral Hypoglycemic Agents on OGTT in Normal rats:

TIME

FBS

0 min

30 min

60min

120 min

 NC

84±1.1

103 .1±1.5


120.6±0.8

(↑16%)

114 .71 ±1.5

(↑10%)

86 .2± 0.6

(↓17%)

 DC

205±2.5

214± 1.4

  

289  ±2.6

(↑35%)

288.3 ±3.0

(↑34.5%)

285.4±2.1

(↑33%)

FLEt

86.3 ±1.20

91 .33± 1.0


108.12± 4.1

(↑18.68%)

104.42±1.9

(↑14%)a

88 .90±0.8

(↓4%)a

  P

85.6 ±1.20

93 .04 ±1.2


120.1±3.4

(↑29%)

112.33±1.6

(↑20%)c

80.8 ±2.8

(↓3%)a

 G

88.20 ±0.57

90.3 ±1.2


116.66 ±3.1

(↑28.8%)

102.12±0.6

(↑13%)a

84.99±0.5

(↓6%)a

P+G

89. 07 ±0.5


90.33± 0.5


106 ±0.5

(↑17.7%)

99 ±0.8

(↑9%)a

70.22± 0.9

(↓23%)a

P+ FLEt

86.1 ±0.5


90.32±0.88


110.9±0.9

( ↑20%)

102 ±1.8

(↑15%)b

85.77± 0.8

(↓5.1% )a

 G+ FLEt

82.66± 0.3

86.66± 1.7


102.8±3.8

(↑15.9%)

96± 2.2

(↑11.6%)a

82.1± 0.7

(↓5%)a

All values are mean ± SEM, n=6,a P<0.001, bP<0.01, cP<0.05 when compared to normal control group.

Table-2. Effect of FLEt and its combination with Oral Hypoglycemic Agents on OGTT in Diabetic rats:

TIME

FBS

0 min

30 min

60min

120 min

 NC

89.26 ±1.2

 98.34± 1.9

    

104.1 ±2.0

  (↑6%)

112.90± 0.9

     (↑15.3%)

90.21± 2.1

  (↑2.8%)

 DC

188.06±3.9

212.11 ±0.8

  

249.2± 0.8

 (↑17.5%)


298.14 ±0.8

 (↑40%)

306.3±0.1

 (↑44%)

FLEt

179.26 ±0.7

189.22±1.5

   

208.9± 3.3

  (↑10.4%)b

222 ±1.2

  (↑17%)a

186.1±1.7

 (↓2%)a

  P

184.43± 0.4

201.66 ±1.2


236.67 ±3.2

 (↑17.4%)c

257.45 ±4.1

  (↑27%)c

212.7±5.0

  (↑5.5%)b

 G

197.26±0.9

203.67±2.1


229.88±2.4

 (↑12.56%)b

243.5±4.3

 (↑20%)a

180±2.9  (↓12.4%)a

P+G

168.8.±0.5


174.29±2.3

   


198.74±0.8

  (↑8%)c


208±1.9

  (↑19%)a

143.1±1.8

  (↓18%)a

P+ FLEt

170.14±1.9

190.2±3.9

    (↑11%)

211.67±4.0

  (↑11%)c

248.15±2.8

   (↑30%)c

194.8±1.8

(↑14%)b

 G+ FLEt

182.88±1.5


189.12±1.7

    

209.37±2.2

   (↑3%)b


219.76±2.1

  ( ↑15%)a

187.4±0.6

   (↓2%)

All values are mean ± SEM, n=6,a P<0.001, bP<0.01, cP<0.05 when compared to diabetic control group.

Table 3: Effect of FLEt and its combination with Oral Hypoglycemics on Blood Glucose (mg/dl)

Groups

 0th day

   15th day

 30th day

45th day

60th day

 75th day

NC


124±0.8

123.2±0.12

126±0.24

128±0.5

122±0.5

123±0.8

DC


265±0.5

273.5±0.2

291.8±0.9

332.8±0.4

395.2±0.5

418±0.4

FLEt


241±0.1

248.4±0.8b

235.8±0.5b

228.4±0.6b

219.4±0.5b

139±0.9a

P


245±0.8

252.5±0.5c

241.2±1.8c

234.6±0.8c

225.8±0.6c

148±0.7a

G


270±0.5

261±0.6c

248.1±2.3c

224.7±0.4c

194.4±0.4c

143±0.4a

P+G


241±0.5

222.8±1.7a

202.5±0.7a

161.1±0.5a

89.1±0.5a

    -

P+FLEt


251±0.7

237.8±0.5b

228.6±1.2b

218.8±0.5b

183.5±1.5b

137±0.1a

G+FLEt


262±0.6

244±0.4a

229.4±0.8b

194.7±0.6a

164.7±0.8a

131±0.1a

FLEt:- Fenugreek leaves exteact.
All values are mean ± SEM, n=6,a P<0.001, bP<0.01, cP<0.05 when compared to    diabetic control group

Table:4  Comparison of relaxant responses in treated groups:

TREATMENT

            Percentage relaxant response to

SNP

                     ACETYLCHOLINE

   0.1 (µM)

     0.3 (µM)

     (1.0 µM)

NORMAL            

              Mean

               SEM


28.35

±3.458


32.37

±3.876


56.56

±4.467


101.00

±1.98

DIABETIC      

              Mean

              ±SEM


9.07

±2.345


14.76

±3.234


20.67

±4.778


103.00

±2.87

FLEt                                       

             Mean

            ±SEM


17.61b

±0.253


21.45b

±0.773


24.88b

±0.197


105.00

±3.12

PIO                     

             Mean

              ±SEM


12.82c

±0.287


16.52c

±0.545


20.01c

±0.345


102.00

±1.87

GLIP            

             Mean

           ±SEM


15.54c

±0.548


17.64c

±0.550


22.98c

±0.791


101.00

±2.34

PIO+GLIP          

              Mean

             ±SEM


16.21b

±0.454


22.81b

±0.657


35.87b

±0.965


103.00

±3.05

FLEt+PIO         

               Mean

              ±SEM


15.20b

±0.505


27.60b

±0.724


37.98b

±0.876


101.00

±1.05

FLEt+GLIP         

             Mean

             ±SEM


16.43b

±0.327


25.70b

±0.479


34.76b

±0.876


103.98

±3.76

FLEt+FLU       

             Mean

            ±SEM


14.54a

±0.347


24.74a

±0.733


36.34a

±0.879


101.00

±1.89

P+FLU

            Mean

            ±SEM


12.86a

±0.291


20.87a

±0.539


32.90a

±0.678


103.00

±2.67

G+FLU

            Mean

            ±SEM


14.70a

±0.305


23.53a

±0.783


38.57a

±0.375


102.00

±2.23

All values are mean ± SEM, n=6,a P<0.001, bP<0.01, cP<0.05 when compared to diabetic control group

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