You are hereMICROSCOPICAL AND PRELIMINARY PHYTOCHEMICAL SCREENING OF ‘Piper betel’
MICROSCOPICAL AND PRELIMINARY PHYTOCHEMICAL SCREENING OF ‘Piper betel’
Fig. No.3. Vein lets present in the upper surface of the leaf
The stomatas present in the 1 sq mm of the leaf were 32.
Fig .No.4.T.S. View of Stomata present in the upper epidermis
The stomata having the more than two guard cells- so it is Anamocytic type stomata
Trans version section of betel leaf:
* It shows that upper, lower epidermal cells and it gives pink colour stain with a drop of phloroglucinol and concentrated hydrochloric shows that presence of lignified cells like xylem and phloem in the vascular bundles of midrib region. And also present prismatic calcium oxalate crystals.
Fig. No.5.T.s view of betel leaf in the mid rib region
* Powder microscopy :the powder microscopy shows that presence of starch grains, calcium oxalate crystals and xylem, phloem
3.2. Preliminary Phytochemical Screening:
Preliminary phytochemical screening: The qualitative chemical tests were performed for the P. betel dried powder according to the methods described by Farnsworth with some modifications.
It shows that presence of alkaloids, tannins, phenolic compounds, flavonoids and steroid glycosides, terpens, anthraquinones, and steroids
Terpenes or terpenoids have been identified as active antiprotozal and antimalarial agents in many pharmacological studies Flavonoids are the other forms of Piper betel phenolic structures. Flavonoids revealed significant anti-parasitic activities against different parasite strains of malaria, trypanosome and leis mania Derivatives of 9, 10-anthraquinone include many important drugs including antimalarials like rufigallol. These chemical compounds which were found in this extract may be acting singly or in synergy with one another to exert the observed antiplasmodial activity of Piper betel.
The present study reveals that the microscopically evidence was strong enough to prove the leaf and the phytochemical screening reports the presence of alkaloids, tannins, phenolic compounds, flavonoids and steroid glycosides, terpenes, anthraquinones, and steroids which may be responsible for various therapeutic activities.
So there is a lot of scope for in vivo studies due to presence of different phytoconstituents.
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